Title:Qingchang Mixture Prevents the Intestinal Ischemia-reperfusion Injury
through TLR4/NF-kB Pathway
Volume: 26
Issue: 1
Author(s): Meng Wang, Yong Zhu, Shujuan Liu, Zhaochun Tian, Pengfei Zhu, Yunjie Zhang*Yongkun Zhou*
Affiliation:
- Department of General Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan
250014, Shandong, China
- Department of General Surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan
250014, Shandong, China
Keywords:
Ischemic and ischemia-reperfusion, Chinese medicine, Qingchang mixture, oxidative stress, inflammatory response, hypoperfusion.
Abstract:
Object: This study aims to determine the protective effect and molecular responses of
the traditional Chinese medicine Qingchang mixture on intestinal ischemia-reperfusion (IR) injury.
Methods: The rat intestinal IR model was prepared. The intestinal ischemic injury was evaluated
by HE staining, biochemical assay and western blot. In addition, a human hypoxia-reoxygenation
(HR) in vitro model was prepared using intestinal epithelial cells (IEC-6). The viability and apoptosis
of IEC-6 cells were measured by CCK8 and apoptosis detection. TAK242 or PDTC was used
as a small molecule inhibitor of TLR4 or NF-κB, respectively.
Results: Compared with the IR group, the pretreatment of the Qingchang mixture reduced the
morphological damage, oxidative stress, inflammatory response, and barrier function damage of
the small intestine tissue. IR significantly increased the expression of TLR4 and NF-κB, while the
pretreatment of the Qingchang mixture inhibited the expression of TLR4 and NF-κB. Furthermore,
the pretreatment of Qingchang mixture, TAK242, or PDTC effectively improved the viability and
hindered apoptosis of the HR-induced IEC-6 cells.
Conclusions: Traditional Chinese medicine Qingchang mixture prevents intestinal IR injury
through TLR4/NF-kB pathway.
