Title:Promising Effects of Naringenin and Melatonin against Hepatic Encephalopathy
Impairments Induced by Bile Duct Ligation in Male Rats
Volume: 22
Issue: 1
Author(s): Elahe Karami Raviz, Fatemeh Noormand, Ali Sharifzadeh Kermani, Ali Galedari, Khadijeh Esmaeilpour, Marzieh Maneshian, Taj Pari Kalantaripour, Shahriar Dabiri and Majid Asadi-Shekaari*
Affiliation:
- Neuroscience Research Center, Neuropharmacology Institute, Kerman University of Medical Sciences, Kerman, Iran
Keywords:
Bile duct ligation, naringenin, melatonin, open field, anti-oxidant, anti-inflammatory, rat.
Abstract:
Background: Bile duct ligation (BDL) is used for evaluating the protective effects of
different agents with anti-inflammatory and antioxidant properties against the liver and brain
damages. Naringenin (N) and melatonin (M) are used as protectants in various models of diseases.
Aims: In the current research, the combinational effects of these well-known anti-inflammatory
and antioxidants agents were investigated against cerebral injuries induced by BDL in male rats.
Methods: The animals were distributed into the following groups: Sham, BDL + Vehicle and
BDL+ N + M. Neuronal damages were evaluated using biochemical, motor behavioral tasks and
morphological assessments.
Results: Based on the data, BDL resulted in decreasing locomotor activity, which was reversed by
N and M. Morphological study confirmed that BDL led to neurodegeneration in the cortex of the
rats, and the N and M treatment preserved cortical neurons. In addition, immunohistochemical
(IHC) study of the rat cortex showed that BDL resulted in increasing the activated astrocytes, and
the N and M treatment reduced the number of activated cells.
Conclusion: These results obviously depicted combinational therapy with N and M to exert positive
effects in the BDL rats, probably due to their synergistic anti-inflammatory and antioxidant
activities.