Title:MiR-129-2-3p Inhibits Esophageal Carcinoma Cell Proliferation, Migration,
and Invasion via Targeting DNMT3B
Volume: 16
Author(s): Xuyang Peng, Xuhui Wu, Gongzhi Wu, Chongxiong Peng, Bin Huang, Mingjiang Huang, Jianyang Ding, Chaofan Mao and Huaizhong Zhang*
Affiliation:
- Department of Cardiothoracic Surgery, The Sixth Affiliated Hospital of Wenzhou Medical University, Lishui, 323000,
China
Keywords:
miR-129-2-3p, DNMT3B, esophageal carcinoma, proliferation, migration, invasion.
Abstract:
Purpose: The study aims to explore the regulatory mechanism of miR-129-2-3p underlying
esophageal carcinoma (EC) cell progression and generate new ideas for targeted treatment of
EC.
Methods: Mature miRNA expression data and total RNA sequencing data of EC in the TCGAESCA
dataset were utilized to explore differentially expressed miRNAs (DEmiRNAs). StarBase database
was then utilized to predict targets of miRNA. MiR-129-2-3p and DNMT3B expression in
EC cell lines was assayed through qRT-PCR and Western blot. CCK-8, scratch healing, and
transwell assays were conducted to assess the impact of miR-129-2-3p on EC cell phenotypes. In
addition, a dual-luciferase assay was completed to identify the binding relationship between
DNMT3B and miR-129-2-3p.
Results: MiR-129-2-3p was noticeably less expressed in EC cell lines, while DNMT3B was highly
expressed. MiR-129-2-3p could bind to DNMT3B. Furthermore, in vitro functional experiments uncovered
that overexpressed miR-129-2-3p repressed EC cell progression while further overexpressing
DNMT3B would restore the above inhibitory effect.
Conclusion: MiR-129-2-3p is a cancer repressor in EC cells, and it could target DNMT3B, thus
hampering the progression of EC cells.
