Title:Contribution of Glutathione-S-Transferases Polymorphism and Risk of
Coronary Artery Diseases: A Meta-Analysis
Volume: 15
Issue: 3
Author(s): Santhi Priya Sobha and Kumar Ebenezar Kesavarao*
Affiliation:
- Faculty of Allied Health Sciences, Chettinad Hospital & Research Institute, Chettinad Academy of Research & Education,
Kelambakkam-603 103, Chengalpattu Dt., TN, India
Keywords:
CAD, GST, gene polymorphism, ethnicity, smoking, meta-analysis, glutathione-s-transferases.
Abstract:
Background: Oxidative stress is one of the risk components in the development of coronary
artery diseases (CAD). Genetic polymorphism in major antioxidant genes like Glutathione-
S-Transferases (GST) has been associated with increased CAD susceptibility and severity.
Objective: To get a precise evaluation and to update the association, a meta-analysis on GST
(GSTM1, GSTT1, and GSTP1) polymorphism with CAD was performed. Moreover, the combined
effect of GSTM1/GSTT1 null genotypes on CAD risk has not yet been studied, but it has the highest
risk of developing diseases.
Materials and Methods: PubMed, Embase, and Web of Science were systematically searched for
eligible studies. Case-control studies in the English language and with genotypic frequency were
selected in order to provide data and calculate the odds ratio (OR). OR with 95% CI was calculated,
and a random effect model was used. NOS scale was used to assess the quality of the included
studies.
Results: Meta-analysis indicated that the GSTM1 null genotype and GSTP1 (Ile105Val) polymorphism
is significantly associated with CAD risk with a pooled OR-1.38, p=0.01 for GSTM1 and
OR-1.19, p=0.04 for GSTP1. The dual null genotype of GSTM1-GSTT1 has the highest risk for
CAD development (OR-1.59, p=0.003), and there is no significant association between GSTT1 null
genotype with CAD. In the subgroup analysis, GSTM1 showed an increased risk for Asians (OR-
1.68, p=<0.01) and smokers (OR-1.98, p=<0.01). Publication bias was not observed.
Conclusion: The findings suggest that the GSTM1 and GSTP1 polymorphism can be a predictive
factor for CAD risk, and a larger sample size is required further to confirm the association.
