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Anti-Cancer Agents in Medicinal Chemistry

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ISSN (Print): 1871-5206
ISSN (Online): 1875-5992

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Research Article

In Vitro Effects of Propofol on Cytotoxic, Apoptotic and PI3K-Akt Signaling Pathway Genes on Brain Cancer Cells

Author(s): Tuba Gokdogan Edgunlu, Cigir Biray Avci*, Neslihan Pınar Ozates, Bakiye Goker Bagca, Sevim Karakas Celik, Aydin Boluk and Bakiye Ugur

Volume 22, Issue 2, 2022

Published on: 30 December, 2021

Page: [356 - 361] Pages: 6

DOI: 10.2174/1871520621666210708094328

Price: $65

Abstract

Aim: The study aimed to determine the cytotoxic and apoptotic effect of propofol on glioma cells.

Background: Propofol [2,6-diisopropylphenol] is a commonly used intravenous anesthetic. Propofol is known to have a mechanism of action on the PI3K-AKT pathway.

Objective: This study aimed to evaluate the effect of propofol on the proliferation and apoptosis of human glioma cells, as well as to investigate changes in expression levels of the PI3K-AKT signaling pathway genes.

Materials and Methods: The cytotoxic effect of propofol on the U-87 MG cell line was determined by WST-1 method. Annexin V-FITC and Mitoprobe JC-1 assay were used to measure apoptosis by flow cytometry. The expression levels of genes in the PI3K-AKT signaling pathway were investigated by qRT-PCR.

Results: We have shown that propofol induced apoptosis in U-87 MG cells by 17.1 fold compared to the untreated control. Furthermore, significant differences were found in the expression levels of the PI3K-AKT signaling pathway genes.

Conclusion: As a result of our study, it was found that propofol caused differences in expression levels of PI3K-AKT signaling pathway genes and it was suggested that these differences may be related to apoptosis induction.

Keywords: Propofol, PI3K-AKT, glioblastoma, gene expression, signaling pathway, apoptosis.

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