Title:Characterization of Metabolites of α-mangostin in Bio-samples from SD Rats by UHPLC-Q-exactive Orbitrap MS
Volume: 22
Issue: 13
Author(s): Fan Dong, Shaoping Wang, Ailin Yang, Haoran Li, Pingping Dong, Bing Wang, Long Dai, Yongqiang Lin*Jiayu Zhang*
Affiliation:
- Shandong Provincial Institute for Food and Drug Control, Shandong Engineering Laboratory for Standard Innovation and Quality Evaluation of TCM, Jinan 250101,China
- School of Pharmacy, BIN ZHOU Medical University, Yantai 260040,China
Keywords:
α-mangostin, metabolism, in vivo, UHPLC-Q-exactive orbitrap MS, xanthones, hydrogenation, sulfation.
Abstract:
Background: α-mangostin, a typical xanthone, often exists in Garcinia mangostana L. (Clusiaceae).
α-mangostin was found to have a wide range of pharmacological properties. However, its specific metabolic
route in vivo remains unclear, while these metabolites may accumulate to exert pharmacological effects, too.
Objective: This study aimed to clarify the metabolic pathways of α-mangostin after oral administration to the rats.
Methods: Here, an UHPLC-Q-Exactive Orbitrap MS was used for the detection of potential metabolites formed
in vivo. A new strategy for the identification of unknown metabolites based on typical fragmentation routes was
implemented.
Results: A total of 42 metabolites were detected, and their structures were tentatively identified in this study.
The results showed that major in vivo metabolic pathways of α-mangostin in rats included methylation,
demethylation, methoxylation, hydrogenation, dehydrogenation, hydroxylation, dehydroxylation,
glucuronidation, and sulfation.
Conclusions: This study is significant to expand our knowledge of the in vivo metabolism of α-mangostin and to
understand the mechanism of action of α-mangostin in rats in vivo.