Title:Impact on Growth and Secondary Metabolites in White Poplar (Populus
alba L.) Callus using SeNPs@Moringa
Volume: 15
Issue: 2
Author(s): Reda E. Abo El-Fadl, Mahmoud M. El-Saber , Manal E.A. Ahmed, Tamer M. Abd Elaziem and Ahmed A. El-Sayed*
Affiliation:
- Department of Photochemistry, National Research Centre, 33 El-Bohouth St., Dokki-12622, Giza, Egypt
Keywords:
White poplar, callus SeNPs, green nanotechnology, secondary metabolite, GCMS, DPPH.
Abstract:
Background: Green synthesis of nanoparticles has provided a cost-effective, environmentally
friendly method, as well as raised safe strategies for the synthesis of nanomaterials.
Methods: In this study, we investigated the synthesis of Selenium nanoparticles from the leaf aqueous
extract of Moringa oleifera (MO-SeNPs) and studied the effect of MO-SeNPs and Moringa extract
with various concentrations on the growth, active constituents, and antioxidant activity (scavenging
DPPH) of Populus alba callus. MS medium supplemented with (2, 4-D at 2 mg/l + 0.5 mg/l
BA) took the maximum callus induction percentage (100%), increasing the callus fresh weight, dry
weight, and degree of callus formation compared with (2.0 mg/l 2,4-D + 0.5 mg/l Kin).
Results: The highest values of callus fresh and dry weight were recorded on MS containing 20%
MO-SeNPs. Regarding the active constituents, the GC-MS chromatogram revealed the presence of
23 major compounds identified in the chloroform extract. The phytoconstituents with maximum
peak area in callus extract are Tetradecane, Hexadecen-1-ol, Trans-9, 5-Octadecene, (Benzene, 1-
pentyloctyl), (Benzene, 1-butylnonyl), Cycloeicosane, and 10-Heneicosene in all treatments.
Moringa extract and MO-SeNPs stimulate the increase of compounds in callus compared to control.
In this regard, MO-SeNPs 5% led to a higher increase in the area percent of active substances compared
to the treatment of Moringa extract and control. Concerning the antioxidant activity, the results
indicated that Moringa extract and MO-SeNPs had a positive effect on scavenging DPPH
compared with the control. The highest percentages of scavenging DPPH were recorded when Populus
alba L. callus treated with MO-SeNPs 20%, 10%, and Moringa extract 20%, percentages
reached (84.65, 75.35, and 71.94%), respectively.
Conclusion: Green synthesis of MO-SeNPs improved secondary metabolites and antioxidant activity
in Populus alba L. callus.
