Title:Porphyromonas gingivalis Lipopolysaccharide-Induced B Cell Differentiation
by Toll-like Receptors 2 and 4
Volume: 29
Issue: 1
Author(s): Sumei Liu, Guojing Liu, Qingxian Luan, Yongping Ma and Xiaoqian Yu*
Affiliation:
- Department of Periodontology, Peking University School and Hospital of Stomatology & National Clinical Research
Center for Oral Diseases & National Engineering Laboratory for Digital and Material Technology of Stomatology &
Beijing Key Laboratory of Digital Stomatology, Beijing 100081, China
Keywords:
P. gingivalis, lipopolysaccharide, interleukin-10, toll-like receptor, Breg cell, periodontitis.
Abstract: Background: Porphyromonas gingivalis (P. gingivalis) is a pathogenic bacterium widely
present in subgingival plaques of patients with periodontitis. It induces periodontitis with bone
loss as its main feature by changing the number and composition of symbiotic microorganisms, as
well as inducing the natural immune response of the host. However, the mechanism of the latter remains
unclear.
Objective: This study aims to investigate the effect of P. gingivalis lipopolysaccharide (LPS) on
regulatory B cells (Breg) in the occurrence and development of periodontitis.
Methods: We detected the mRNA levels of IL-10 in B cells under the stimulation of P. gingivalis
LPS and/or E. coli LPS, distinguished IL-10-producing cells from different B cell subgroups using
flow cytometry. Through toll-like receptor (TLR) knockout mice, the role of TLR2 and TLR4 in
this process was also evaluated.
Results: Results showed that P. gingivalis stimulated B cells to produce IL-10 via TLR2/4.
CD5+B1 subset is the main source of IL-10+Breg cell. Under P. gingivalis LPS stimulation,
CD5+IgM+CD93-IL-10+B cell subset increased significantly, which was regulated through TLR2/
4.
Conclusion: The results of this study provides new insights into the immunopathogenic mechanism
of P. gingivalis, preliminarily discussed the effect of P. gingivalis on the production of Breg,
and present a theoretical foundation for subsequent investigations on the occurrence and development
of periodontitis.