Regeneration Potential of Bone Marrow-derived Mesenchymal Stromal Cells Lysate for H₂O₂ (In-Vitro) Injured Cells

Title:Regeneration Potential of Bone Marrow-derived Mesenchymal Stromal Cells Lysate for H₂O₂ (In-Vitro) Injured Cells

Volume: 10 Issue: 2

Author(s): Sabeen Malik, Sana Javaid Awan*, Mariam Hashim, Anam Farzand and Shumaila Nadeem

Affiliation:

• Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan

Keywords: Stem cells, bone marrow-derived mesenchymal stromal cells (BMSCs), lysate, regeneration, H2O2, cell-free therapy, ELISA, APX.

Abstract: Background: Mesenchymal stromal cells (MSCs) and their paracrine factors make them a suitable cell-free-therapeutic candidate. Cellular lysate usage could be an effective treatment strategy that circumvents the possible associated drawbacks of stem cell therapies.

Objective: Thus, this research aims to examine the injury regeneration potential of MSCs cellular lysate derived from bone marrow by studying its anti-apoptotic, proliferative, and anti-oxidative effects.

Methods: Hydrogen peroxide (H₂O₂) was used to induce cellular injury. MTT assay, trypan blue, and crystal violet assays were used to assess bone marrow-derived mesenchymal stromal cell (BMSCs) lysate treated cells' viabilities. Next, to investigate the BMSCs lysate anti-oxidative potential anti-oxidants, ascorbate peroxidase (APX), glutathione reductase (GR), and superoxide dismutase (SOD) assays were performed. Simultaneously, the proliferative and anti-apoptotic potential was measured via vascular endothelial growth factor (VEGF A) and p53 expression analysis through immunostaining and ELISA.

Results: It was observed that BMSCs lysate enhances the viability of H₂O₂ injured cells. APX, GR, and SOD's results indicated that after H₂O₂ injury, the anti-oxidant status decreased significantly and was uplifted by BMSCs lysate treatment. Additionally, the results of p53, BAX, and caspase-3 expression revealed that BMSCs lysate inhibits apoptosis by downregulating their expression in treated cells. The VEGF protein expression findings demonstrated that BMSCs lysate upregulates the downregulated expression of VEGF in H₂O₂ injured cells. The expression of proliferative markers (TOP2A, PCNA, and Ki-67) was also elevated in BMSCs treated cells.

Conclusion: To conclude this study's findings, it was observed that BMSCs lysate could decrease H₂O₂ injury and possibly regenerate the injured cells by enhancing their viability and proliferation, improving anti-oxidants levels, and alleviating apoptosis.

Cite this article as:

Malik Sabeen , Awan Javaid Sana*, Hashim Mariam , Farzand Anam and Nadeem Shumaila , Regeneration Potential of Bone Marrow-derived Mesenchymal Stromal Cells Lysate for H₂O₂ (In-Vitro) Injured Cells, Current Biotechnology 2021; 10 (2) . https://dx.doi.org/10.2174/2211550110666210908111751