Title:In Silico Docking of Novel Phytoalkaloid Camalexin in the Management of
Benomyl Induced Parkinson's Disease and its In Vivo Evaluation by Zebrafish
Model
Volume: 21
Issue: 4
Author(s): Tamilanban Thamaraikani, Manasa Karnam*Chitra Velapandian
Affiliation:
- Department of Pharmacology, SRM College of Pharmacy,SRMIST,Kattankulathur-603203,Tamilnadu, India
Keywords:
Camalexin, Autodock 4.2, Alda-1, aldehyde dehydrogenase, benomyl, Parkinson’s disease.
Abstract: Background: Parkinson’s Disease (PD) exhibits the extrapyramidal symptoms caused
due to the dopaminergic neuronal degeneration in the substantia nigra of the brain and depletion of
Aldehyde Dehydrogenase (ALDH) enzyme.
Objective: This study was designed to enlighten the importance of the Aldehyde dehydrogenase enzyme
in protecting the dopamine levels in a living system. Camalexin, a potentially active compound,
has been evaluated for its dopamine enhancing and aldehyde dehydrogenase protecting role
in pesticide-induced Parkinson’s disease.
Methods: AutoDock 4.2 software was employed to perform the docking simulations between the ligand
camalexin and standard drugs Alda-1, Ropirinole with three proteins 4WJR, 3INL, 5AER.
Consequently, the compound was evaluated for its in vivo neuroprotective role in the zebrafish
model by attaining Institutional Animal Ethical Committee permission. The behavioral assessments
and catecholamine analysis in zebrafish were performed.
Results: The Autodock result shows that the ligand camalexin has a lower binding energy (-3.84)
that indicates a higher affinity with the proteins when compared to the standard drug of proteins
(-3.42). In the zebrafish model, behavioral studies provided evidence that camalexin helps in the
improvement of motor functions and cognition. The catecholamine assay has proved that there is
an enhancement in dopamine levels, as well as an improvement in aldehyde dehydrogenase enzyme.
Conclusion: The novel compound, camalexin, offers a protective role in Parkinson’s disease model
by its interaction with neurochemical proteins and also in alternative in vivo model.