Title:Cascade Biocatalytic Processes through Combined Cross-linked Enzyme Aggregates
Volume: 25
Issue: 21
Author(s): Nicoletta Gaggero*Domenico C.M. Albanese
Affiliation:
- Dipartimento di Scienze Farmaceutiche, Sezione di Chimica Generale e Organica “A. Marchesini”, Universita degli Studi di Milano, Milano,Italy
Keywords:
CLEA; Combi-CLEAs, enzyme cascades, biotransformation, biocatalysis, immobilized enzymes, green chemistry, multienzymatic
system.
Abstract: Cascade reactions catalyzed by immobilized multi enzymatic systems are emerging
as a tool to address the increasing need for green and sustainable chemistry. In this regard, the
carrier-free immobilization strategy of combined cross-linked enzymes aggregates (combi-
CLEAs) is of great interest. In a combi-CLEAs two or more enzymes are co-immobilized into
a single unit where they are accommodated in close proximity in order to minimize the intermediates
diffusion in the reaction medium, at the same time allowing the access of substrates
and coenzymes to the active sites. The use of CLEAs provides a simple and low cost methodology
that enhances operational and storage enzyme stability and facilitates biocatalyst recovery
and recycle. Some significant applications of cascade reactions catalyzed by combi-
CLEAs are reported in this mini-review. These examples have been applied on a multigram
scale and have furnished practical steps towards the implementation of enzymes on an industrial scale.
