Title:PEGylated DPPC/Anti-SNAP25 Antibody Targeted Liposomes from Langmuir Monolayer Study to Formulations
Volume: 15
Issue: 3
Author(s): Lai Ti Gew*Misni Misran
Affiliation:
- Department of Biological Sciences, School of Medical and Life Sciences, Sunway University, Subang Jaya, Malaysia
Keywords:
Intermolecular interactions, membrane fluidity, drug delivery, stealth liposomes, DPPC/Anti-SNAP25 antibody, Langmuir-Blodgett (LB).
Abstract: Background: Molecule compatibility is an important factor to be considered before preparing
antibody-targeted liposomes, stealth-liposomes, and stealth antibody-targeted liposomes.
Objective: To determine the intermolecular interaction of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamide-
N-[methoxy(polyethyleneglycol)-2000] (ammonium salt), DOPE PEG2000 and Anti-SNAP25
(AS25) in 1,2-dipalmitoyl-sn-glycero-3-phosphorylcholine (DPPC) monolayer, and their liposomes.
Methods: In this study, DPPC was used to create a monolayer mimicking the half membrane of liposomes
to investigate its interactions with a polyclonal antibody, AS25, and DOPE PEG2000,
which are based on Langmuir-Blodgett (LB) techniques. The surface morphology of DPPC-AS25
and DPPC-DOPE PEG2000-AS25 bilayers were also imaged and analyzed by using atomic force
microscopy (AFM) to support the LB findings. The LB findings were then utilized as a reference
to prepare DPPC liposomes in this work.
Results: The best mole ratio of DPPC-DOPE PEG2000, determined to be 50 to 1, was used to
study the interaction with the polyclonal antibody AS25. The free energy of mixing (ΔGmix) of DPPC-
DOPE PEG2000-AS25 was more negative than DPPC-AS25 in the entire investigated ranges,
indicating that the ternary mixture of DPPC-DOPE PEG2000-AS25 was more compatible than the
binary mixture of DPPC-AS25. The presence of DOPE PEG2000 in DPPC-AS25 increased the
fluidity of the membrane, which resulted in a greater interaction of AS25 with DPPC.
Conclusion: The constant values of particle size and zeta potential measurements of DPPC-DOPE
PEG2000-AS25 liposomes showed agreement with the LB findings, indicating that LB is a good
technique to predict precise liposomal formulations.
