Title:Del-1 in Psoriasis Induced the Expression of αvβ3 and α5β1 in
Endothelial Cells
Volume: 22
Issue: 5
Author(s): Xuping Niu, Qixin Han, Xiaofang Li, Juan Li, Yanmin Liu, Yan Li and Yan Wu*
Affiliation:
- Department of Dermatology, The First Hospital of China Medical University, Shenyang, China
Keywords:
Psoriasis, Del-1, dermal mesenchymal stem cells (dMSCs), integrin, angiogénesis, western blotting.
Abstract:
Objective: Psoriasis is a chronic inflammatory skin disease highly depending
on angiogenesis. Our prior results showed that the mRNA and protein of Del-1 in dermal
mesenchymal stem cells (dMSCs) was up-regulated from psoriasis. Our aim was further
to investigate the role of Del-1 from dMSCs in the pathogenesis of psoriasis and confirm
the effect of Del-1 on the pathogenesis of psoriasis.
Methods: We conducted an immunohistochemistry experiment to further investigate the
expression of Del-1in psoriatic lesions. In addition, dMSCs with over-expressed Del-1
via the lentiviral vector of Del-1 were co-cultured with ECs, and the protein expression of
integrins (αvβ3, αvβ5 ,and α5β1) of ECs were detected by western blotting.
Results: This research showed that Del-1 was significantly increased in lesions of
patients with psoriasis (p< .05, 9.96 vs. 2.18), and Del-1 from dMSCs successfully
induced up-regulation of integrins α5β1 and αvβ3 (all p < .05).
Conclusion: This study demonstrated that Del-1 from dMSCs was involved in the
pathogenesis of psoriasis through induced angiogenesis. And Del-1, αvβ3 and α5β1
may be potential new targets for inhibiting angiogenesis in psoriasis.
