Title:Scaffolds Designing from Protein-loadable Coaxial Electrospun Fibermats of poly(acrylamide)-Co-poly(diacetone acrylamide) and Gelatin
Volume: 4
Issue: 2
Author(s): Yuji Tanikawa, Akiko Obata, Kenji Nagata, Toshihiro Kasuga and Toshihisa Mizuno*
Affiliation:
- Department of Life Science and Applied Chemistry, Graduate School of Engineering, Nagoya Institute of Technology, Gokiso-Cho, Showa-Ku, Nagoya, Aichi 466-8555,Japan
Keywords:
Fibermat, co-axial electrospinning, protein-encapsulation, gelatin, scaffold, growth factor, CLG shell layer.
Abstract:
Background: Aiming at in situ regenerative therapy, the tailored design of cytokine-releasing
scaffolds is still one of the crucial issues to be studied. A core-shell fibermat is one of the attractive
platforms for this purpose. But, very few detail the importance of choosing the right material
for the shell units that can endow efficient release properties.
Objective: In this study, we characterized the effectiveness of core-shell fibermats that possess
cross-linked gelatin (CLG) as the shell layer of constituent nanofibers, as a protein-releasing cell-incubation
scaffold.
Methods: For the core nanofibers in the core-shell fibermats, we utilized a crosslinked copolymer
of poly(acrylamide)-co-poly(diacetone acrylamide) (poly(AM/DAAM)) and adipic acid dihydrazide
(ADH), poly(AM/DAAM)/ADH. By coaxial electrospinning and the subsequent crosslinking
of the gelatin layer, we successfully constructed core-shell fibermats consisting of double-layered
nanofibers of poly(AM/DAAM)/ADH and CLG. Using fluorescein isothiocyanate-labeled lysozyme
(FITC-Lys) as a dummy guest protein, we characterized the release behavior of the coreshell
fibermats containing a CLG layer. Upon loading basic fibroblast growth factor (bFGF) as cargo
in our fibermats, we also characterized impacts of the released bFGF on proliferation of the incubated
cells thereon.
Results: Although the single-layered poly(AM/DAAM)/ADH nanofiber fibermats did not adhere
to the mammalian cells, the core-shell fibermat with the CLG shell layer exhibited good adherence
and subsequent proliferation. A sustained release of the preloaded FITC-Lys over 24 days without
any burst release was observed, and the cumulative amount of released protein reached over 65%
after 24 days. Upon loading bFGF in our fibermats, we succeeded in promoting cell proliferation,
and highlighting its potential for use in therapeutic applications.
Conclusion: We successfully confirmed that core-shell fibermats with a CLG shell layer around
the constituent nanofibers, were effective as protein-releasing cell-incubation scaffolds.
