Title:Mesenchymal Stem Cells Lysate as a Cell-Free Therapy: A Review
Volume: 10
Issue: 2
Author(s): Sabeen Malik*Tehreem Malik
Affiliation:
- Institute of Molecular Biology and Biotechnology (IMBB), The University of Lahore, Lahore, Pakistan
Keywords:
Stem cells, cell lysate, mesenchymal stem cells (MSCs), cell-free therapy, bioactive factors, regenerative medicine.
Abstract: Abstract: Mesenchymal Stem Cells (MSCs), a self-renewing and multipotent population of cells
have proved to be a potential regenerative medical therapy option. Initially, it was assumed that the
primary mechanism behind MSCs action was cell substitution by differentiation and engraftment at
the injury site. However, recent trials have demonstrated that implanted cells don’t last long and
that the benefits of MSCs therapy may be triggered by a wide number of bioactive factors, essential
for regulating main biological processes. Thus, supporting the paracrine mechanism of action
of MSCs. Application of secretome could eliminate the potential risk factors associated with MSCs
transplantation, like the unknown fate of cells after transplantation, malignant transformation, and
undesired differentiation of MSCs. However, the use of a secretome might not give long-lasting
and desired results. Thus, it may intuitively suggest the need for other treatment options. The cellular
lysate is considered as an alternate cell-free treatment strategy for the cure of multiple disorders.
The present review describes the MSCs secretome/extracellular vesicles and MSCs lysate, derived
from bone marrow and adipose, as a cell-free therapy with the application of MSCs lysate for various
diseases from 2010 to 2020. Moreover, it also highlights the advantages and limitations of each
cell-free therapy.
