Title:Effects of Macroporous Resin Extract of Dendrobium officinale Leaves in
Rats with Hyperuricemia Induced by Fructose and Potassium Oxonate
Volume: 25
Issue: 8
Author(s): Yu-Zhi Wang, Cong Zhou, Li-Jie Zhu, Xing-Li-Shang He, Lin-Zi Li, Xiang Zheng, Wan-Feng Xu, Ying-Jie Dong, Bo Li, Qiao-Xian Yu*, Gui-Yuan Lv*Su-Hong Chen*
Affiliation:
- Zhejiang Senyu Co. Ltd., Yiwu, Zhejiang 322099, China
- College of
Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, China
- Collaborative Innovation Center of Yangtze River Delta Region Green Pharmaceuticals, Zhejiang University of
Technology, Hangzhou, Zhejiang 310014, China
Keywords:
Dendrobium officinale leaves, hyperuricemia, fructose, urate transporter, xanthine oxidase, GLUT9.
Abstract:
Aims and Objectives: Fructose, as a ubiquitous monosaccharide, can promote ATP
consumption and elevate circulating Uric Acid (UA) levels. Our previous studies have confirmed that
the macroporous resin extract of Dendrobium officinale leaves (DoMRE) could reduce the UA level of
rats with hyperuricemia induced by a high-purine diet. This study aimed to investigate whether
DoMRE had a UA-lowering effect on rats with hyperuricemia caused by fructose combined with
potassium oxonate, so as to further clarify the UA-lowering effect of DoMRE, and to explore the UAlowering
effect of DoMRE on both UA production and excretion.
Materials and Methods: Rats with hyperuricemia induced by fructose and potassium oxonate were
administered with DoMRE and vehicle control, respectively, to compare the effects of the drugs. At
the end of the experiment, the Serum Uric Acid (SUA) and Creatinine (Cr) levels were measured using
an automatic biochemical analyzer, the activities of xanthine oxidase (XOD) were measured using an
assay kit, and the protein expressions of Urate Transporter 1 (URAT1), glucose transporter 9
(GLUT9), and ATP-Binding Cassette Superfamily G member 2 (ABCG2) were assessed using
immune-histochemical and western blot analyses. Hematoxylin and eosin staining was used to assess
the histological changes in the kidney, liver, and intestine.
Results: Fructose and potassium induced hyperuricemia in rats. Meanwhile, the activities of XOD
were markedly augmented, the expression of URAT1 and GLUT9 was promoted, and the expression
of ABCG2 was reduced, which were conducive to the elevation of UA. However, exposure to DoMRE
reversed these fructose- and potassium oxonate-induced negative alternations in rats. The activities of
XOD were recovered to the normal level, reducing UA formation; the expressions of URAT1,
ABCG2, and GLUT9 returned to the normal level, resulting in an increase in renal urate excretion.
Conclusion: DoMRE reduces UA levels in rats with hyperuricemia induced by fructose combined with
potassium oxonate by inhibiting XOD activity and regulating the expression of ABCG2, URAT1, and
GLUT9. DoMRE is a potential therapeutic agent for treating hyperuricemia through inhibiting UA
formation and promoting UA excretion.