Association of Val16Ala Polymorphism of Manganese Superoxide Dismutase
(MnSOD) with Food Intake and Cardiometabolic Risk Factors in
the Elderly in Primary Care in Porto Alegre

Maria   G.V.   Gottlieb; Cristiane   A.   Borges; Vera   E.   Closs; Raquel       Seibel; Rodolfo   H.   Schneider; Carla   H.   Schwanke; Irenio      Gomes

doi:10.2174/1874609814666210526115150

Abstract

Background: The aging process causes physiological changes on its own. The combination of an unhealthy lifestyle with the presence of genetic polymorphisms, such as the Val16Ala of the antioxidant enzyme manganese-dependent superoxide dismutase (MnSOD) may contribute to a greater occurrence of cardiometabolic risk factors.

Objective: This study aimed to verify the association of Val16Ala-MnSOD polymorphism with food intake, caloric expenditure, and cardiometabolic risk factors in the elderly.

Methods: A cross-sectional study with a sample size of 270 elderly individuals assisted in primary health care in the city of Porto Alegre, RS, Brazil. Val16Ala polymorphism, glucose, lipid profile, insulin, HOMA-IR, blood pressure, waist circumference, PCR-us, IL-6, food consumption, and caloric expenditure were evaluated.

Results: The average age of the elderly was 68.6 ± 7.6 years. There were statistically significant differences regarding the consumption of two or more servings of fruits and vegetables daily between the elderly VV versus AV (P=0.017). There were also statistically significant differences regarding the consumption of two or more daily servings of legumes and eggs between the elderly AA versus VV (P=0.002). The median of insulin was higher in the elderly AA versus AV (P=0.025) and the median of HOMA-IR was higher in the elderly VV versus AV (P=0.029). AA elderly individuals had higher means of high-density lipoprotein (HDL-c), compared to AV (P=0.029).

Conclusion: The results suggest that Val16Ala -MnSOD polymorphism is associated with the consumption of fruits, vegetables, legumes, and eggs, as well as with cardiometabolic risk factors in the elderly.

Keywords: Aging, superoxide dismutase, genetic polymorphism, inflammation, food consumption, energy metabolism.

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[1] 
Ackermann M, Chao L, Bergstrom CT, Doebeli M. On the evolutionary origin of aging. Aging Cell  2007; 6(2): 235-44.
[http://dx.doi.org/10.1111/j.1474-9726.2007.00281.x] [PMID:  17376147] 
[2] 
Fabian D, Flatt T. The evolution of aging. Nat Edu Knowledge  2011; 3(10): 9.
[3] 
Afanas’ev I. Signaling and damaging functions of free radicals in aging-free radical theory, hormesis, and TOR. Aging Dis  2010; 1(2): 75-88.
[PMID:  22396858] 
[4] 
Davidovic M, Sevo G, Svorcan P, Milosevic DP, Despotovic N, Erceg P. Old age as a privilege of the “selfish ones”. Aging Dis  2010; 1(2): 139-46.
[PMID:  22396861] 
[5] 
Finch CE. Longevity, senescence and the genome. Chicago, IL: University of Chicago Press 1990.
[6] 
Kirkwood TB, Austad SN. Why do we age? Nature  2000; 408(6809): 233-8.
[http://dx.doi.org/10.1038/35041682] [PMID:  11089980] 
[7] 
Pourvali K, Abbasi M, Mottaghi A. Role of superoxide dismutase 2 gene Ala16Val polymorphism and total antioxidant capacity in diabetes and its complications. Avicenna J Med Biotechnol  2016; 8(2): 48-56.
[PMID:  27141263] 
[8] 
Banerjee M, Vats P. Reactive metabolites and antioxidant gene polymorphisms in Type 2 diabetes mellitus. Redox Biol  2014; 2: 170-7.
[http://dx.doi.org/10.1016/j.redox.2013.12.001] [PMID:  25460725] 
[9] 
Bresciani G, Cruz IB, de Paz JA, Cuevas MJ, González-Gallego J. The MnSOD Ala16Val SNP: relevance to human diseases and interaction with environmental factors. Free Radic Res  2013; 47(10): 781-92.
[http://dx.doi.org/10.3109/10715762.2013.836275] [PMID:  23952573] 
[10] 
Barbisan F, Azzolin VF, Ribeiro EE, Duarte MMMF, da Cruz IBM. The in vitro influence of a genetic superoxide-hydrogen peroxide imbalance on immunosenescence. Rejuvenation Res  2017; 20(4): 334-45.
[http://dx.doi.org/10.1089/rej.2016.1892] [PMID:  28285577] 
[11] 
Kakkoura MG, Demetriou CA, Loizidou MA, et al. MnSOD and CAT polymorphisms modulate the effect of the Mediterranean diet on breast cancer risk among Greek-Cypriot women. Eur J Nutr  2016; 55(4): 1535-44.
[http://dx.doi.org/10.1007/s00394-015-0971-5] [PMID:  26130326] 
[12] 
Li JY, Tao F, Wu XX, Tan YZ, He L, Lu H. Polymorphic variations in manganese superoxide dismutase (MnSOD) and endothelial nitric oxide synthase (eNOS) genes contribute to the development of type 2 diabetes mellitus in the Chinese Han population. Genet Mol Res  2015; 14(4): 12993-3002.
[http://dx.doi.org/10.4238/2015.October.21.20] [PMID:  26505452] 
[13] 
Minlikeeva AN, Browne RW, Ochs-Balcom HM, et al. Single-nucleotide polymorphisms and markers of oxidative stress in healthy women. PLoS One  2016; 11(6)e0156450
[http://dx.doi.org/10.1371/journal.pone.0156450] [PMID:  27271305] 
[14] 
Roy N, Dasgupta D, Mukhopadhyay I, et al. Genetic association and gene-gene interaction reveal genetic variations in ADH1B, GSTM1 and MnSOD independently confer risk to alcoholic liver diseases in India. PLoS One  2016; 11(3)e0149843
[http://dx.doi.org/10.1371/journal.pone.0149843] [PMID:  26937962] 
[15] 
Taufer M, Peres A, de Andrade VM, et al. Is the Val16Ala manganese superoxide dismutase polymorphism associated with the aging process? J Gerontol A Biol Sci Med Sci  2005; 60(4): 432-8.
[http://dx.doi.org/10.1093/gerona/60.4.432] [PMID:  15933380] 
[16] 
Förstermann U. Oxidative stress in vascular disease: Causes, defense mechanisms and potential therapies. Nat Clin Pract Cardiovasc Med  2008; 5(6): 338-49.
[http://dx.doi.org/10.1038/ncpcardio1211] [PMID:  18461048] 
[17] 
Duarte MM, Moresco RN, Duarte T, et al. Oxidative stress in hypercholesterolemia and its association with Ala16Val superoxide dismutase gene polymorphism. Clin Biochem  2010; 43(13-14): 1118-23.
[http://dx.doi.org/10.1016/j.clinbiochem.2010.07.002] [PMID:  20627099] 
[18] 
Duarte T, da Cruz IB, Barbisan F, Capelleto D, Moresco RN, Duarte MM. The effects of rosuvastatin on lipid-lowering, inflammatory, antioxidant and fibrinolytics blood biomarkers are influenced by Val16Ala superoxide dismutase manganese-dependent gene polymorphism. Pharmacogenomics J  2016; 16(6): 501-6.
[http://dx.doi.org/10.1038/tpj.2015.91] [PMID:  26882122] 
[19] 
Flores AE, Pascotini ET, Kegler A, et al. ALA16VAL-MnSOD gene polymorphism and stroke: Association with dyslipidemia and glucose levels. Gene  2017; 627: 57-62.
[http://dx.doi.org/10.1016/j.gene.2017.05.055] [PMID:  28552711] 
[20] 
Montano MA, Lera JPB, Gottlieb MG, et al. Association between manganese superoxide dismutase (MnSOD) gene polymorphism and elderly obesity. Mol Cell Biochem  2009; 328(1-2): 33-40.
[http://dx.doi.org/10.1007/s11010-009-0071-z] [PMID:  19262996] 
[21] 
Borges CA, Closs VE, Moresco RN, Jacondino CB, da Silva Filho IG, Gottlieb MGV. MnSOD Val16Ala gene polymorphism is associated with REDOX biomarkers in the elderly of primary health care in the city of Porto Alegre. Free Radic Res  2020; 54(5): 293-300.
[http://dx.doi.org/10.1080/10715762.2020.1760263] [PMID:  32326766] 
[22] 
Fan AZ, Yesupriya A, Chang MH, et al. Gene polymorphisms in association with emerging cardiovascular risk markers in adult women. BMC Med Genet  2010; 11: 6.
[http://dx.doi.org/10.1186/1471-2350-11-6] [PMID:  20078877] 
[23] 
Anderson AL, Harris TB, Houston DK, et al. Relationships of dietary patterns with body composition in older adults differ by gender and PPAR-γ Pro12Ala genotype. Eur J Nutr  2010; 49(7): 385-94.
[http://dx.doi.org/10.1007/s00394-010-0096-9] [PMID:  20174813] 
[24] 
Zhang F, Lewis M, Yang G, Iriondo-Perez J, Zeng Y, Liu J. Apolipoprotein E polymorphism, life stress and self-reported health among older adults. J Epidemiol Community Health  2008; 62(4)e3
[http://dx.doi.org/10.1136/jech.2007.063917] [PMID:  18365330] 
[25] 
Alsaleh A, Crepostnaia D, Maniou Z, et al. Adiponectin gene variant interacts with fish oil supplementation to influence serum adiponectin in older individuals. J Nutr  2013; 143(7): 1021-7.
[http://dx.doi.org/10.3945/jn.112.172585] [PMID:  23658423] 
[26] 
Paula RS, Souza VC, Benedet AL, et al. Dietary fat and apolipoprotein genotypes modulate plasma lipoprotein levels in Brazilian elderly women. Mol Cell Biochem  2010; 337(1-2): 307-15.
[http://dx.doi.org/10.1007/s11010-009-0313-0] [PMID:  19915960] 
[27] 
Miranda-Vilela AL, Alves PC, Akimoto AK, Pereira LC. Klautau-Guimarães MdN, Grisolia CK. The effect of hydrogen peroxide-induced oxidative stress on leukocytes depends on age and physical training in healthy human subjects carrying the same genotypes of antioxidant enzymes’ gene polymorphisms. Am J Hum Biol  2010; 22(6): 807-12.
[http://dx.doi.org/10.1002/ajhb.21086] [PMID:  20878976] 
[28] 
Mathieu E, Bernard AS, Delsuc N, et al. A cell-penetrant manganese superoxide dismutase (MnSOD) mimic is able to complement MnSOD and exerts an antiinflammatory effect on cellular and animal models of inflammatory bowel diseases. Inorg Chem  2017; 56(5): 2545-55.
[http://dx.doi.org/10.1021/acs.inorgchem.6b02695] [PMID:  28198622] 
[29] 
Wu S, Yano S, Chen J, et al. Polyphenols from lonicera caerulea L. Berry inhibit LPS-induced inflammation through dual modulation of inflammatory and antioxidant mediators. J Agric Food Chem  2017; 65(25): 5133-41.
[http://dx.doi.org/10.1021/acs.jafc.7b01599] [PMID:  28573848] 
[30] 
Falone S, Santini SJ, Cordone V, et al. Regular and moderate exercise counteracts the decline of antioxidant protection but not methylglyoxal-dependent glycative burden in the ovary of reproductively aging mice. Oxid Med Cell Longev  2016; 20163837623
[http://dx.doi.org/10.1155/2016/3837623] [PMID:  28116035] 
[31] 
Wong HS, Dighe PA, Mezera V, Monternier PA, Brand MD. Production of superoxide and hydrogen peroxide from specific mitochondrial sites under different bioenergetic conditions. J Biol Chem  2017; 292(41): 16804-9.
[http://dx.doi.org/10.1074/jbc.R117.789271] [PMID:  28842493] 
[32] 
Jakicic JM, Clark K, Coleman E, et al. American College of Sports Medicine position stand. Appropriate intervention strategies for weight loss and prevention of weight regain for adults. Med Sci Sports Exerc  2001; 33(12): 2145-56.
[http://dx.doi.org/10.1097/00005768-200112000-00026] [PMID:  11740312] 
[33] 
Gomes I, Nogueira EL, Engroff P, Ely LS, Schwanke CHA, Carli GAD. Resende TdL. The multidimensional study of the elderly in the family health strategy in Porto Alegre, Brazil (EMI-SUS). PAJAR  2013; 1(1): 20-4.
[34] 
Sposito AC, Caramelli B, Fonseca FA, et al. IV brazilian guideline for dyslipidemia and atherosclerosis prevention: Department of atherosclerosis of brazilian society of cardiology. Arq Bras Cardiol  2007; 88(Suppl. 1): 2-19.
[http://dx.doi.org/10.1590/S0066-782X2007000700002] [PMID:  17515982] 
[35] 
National Cholesterol Education Program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III). Third report of the national cholesterol education program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III) final report. Circulation  2002; 106(25): 3143-421.
[http://dx.doi.org/10.1161/circ.106.25.3143] [PMID:  12485966] 
[36] 
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem  1972; 18(6): 499-502.
[http://dx.doi.org/10.1093/clinchem/18.6.499] [PMID:  4337382] 
[37] 
 SBD. Diretrizes da sociedade brasileira de diabetes: 2014-2015. são
	paulo: Sociedade Brasileira de Diabete. 2015.
[38] 
Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC. Homeostasis model assessment: Insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia  1985; 28(7): 412-9.
[http://dx.doi.org/10.1007/BF00280883] [PMID:  3899825] 
[39] 
Lahiri DK, Nurnberger JI Jr. A rapid non-enzymatic method for the preparation of HMW DNA from blood for RFLP studies. Nucleic Acids Res  1991; 19(19): 5444.
[http://dx.doi.org/10.1093/nar/19.19.5444] [PMID:  1681511] 
[40] 
Ambrosone CB, Freudenheim JL, Thompson PA, et al. Manganese superoxide dismutase (MnSOD) genetic polymorphisms, dietary antioxidants, and risk of breast cancer. Cancer Res  1999; 59(3): 602-6.
[PMID:  9973207] 
[41] 
Lustosa LP, Pereira DS, Dias RC, Britto RR, Parentoni AN, Pereira LSM. Tradução e adaptação transcultural do Minnesota Leisure Time Activities Questionnaire em idosos. Geriatria & Gerontologia  2011; 5(2): 57-65.
[42] 
Taylor HL, Jacobs DR Jr, Schucker B, Knudsen J, Leon AS, Debacker G. A questionnaire for the assessment of leisure time physical activities. J Chronic Dis  1978; 31(12): 741-55.
[http://dx.doi.org/10.1016/0021-9681(78)90058-9] [PMID:  748370] 
[43] 
Ainsworth BE, Haskell WL, Leon AS, et al. Compendium of physical activities: classification of energy costs of human physical activities. Med Sci Sports Exerc  1993; 25(1): 71-80.
[http://dx.doi.org/10.1249/00005768-199301000-00011] [PMID:  8292105] 
[44] 
Guigoz Y, Vellas B, Garry PJ. Mini Nutritional Assessment: A practical assessment tool for grading the nutritional state of elderly patients.In: Paris: Serdi: Facts and research in gerontology 1994;
	pp 15-59. 
[45] 
Rosenblum JS, Gilula NB, Lerner RA. On signal sequence polymorphisms and diseases of distribution. Proc Natl Acad Sci USA  1996; 93(9): 4471-3.
[http://dx.doi.org/10.1073/pnas.93.9.4471] [PMID:  8633092] 
[46] 
Sutton A, Khoury H, Prip-Buus C, Cepanec C, Pessayre D, Degoul F. The Ala16Val genetic dimorphism modulates the import of human manganese superoxide dismutase into rat liver mitochondria. Pharmacogenetics  2003; 13(3): 145-57.
[http://dx.doi.org/10.1097/00008571-200303000-00004] [PMID:  12618592] 
[47] 
Crawford A, Fassett RG, Geraghty DP, et al. Relationships between single nucleotide polymorphisms of antioxidant enzymes and disease. Gene  2012; 501(2): 89-103.
[http://dx.doi.org/10.1016/j.gene.2012.04.011] [PMID:  22525041] 
[48] 
Grasbon-Frodl EM, Kösel S, Riess O, Müller U, Mehraein P, Graeber MB. Analysis of mitochondrial targeting sequence and coding region polymorphisms of the manganese superoxide dismutase gene in German Parkinson disease patients. Biochem Biophys Res Commun  1999; 255(3): 749-52.
[http://dx.doi.org/10.1006/bbrc.1998.9998] [PMID:  10049782] 
[49] 
Stoehlmacher J, Ingles SA, Park DJ, Zhang W, Lenz HJ. The -9Ala/-9Val polymorphism in the mitochondrial targeting sequence of the manganese superoxide dismutase gene (MnSOD) is associated with age among Hispanics with colorectal carcinoma. Oncol Rep  2002; 9(2): 235-8.
[http://dx.doi.org/10.3892/or.9.2.235] [PMID:  11836586] 
[50] 
Van Landeghem GF, Tabatabaie P, Beckman G, Beckman L, Andersen PM. Manganese-containing superoxide dismutase signal sequence polymorphism associated with sporadic motor neuron disease. Eur J Neurol  1999; 6(6): 639-44.
[http://dx.doi.org/10.1046/j.1468-1331.1999.660639.x] [PMID:  10529750] 
[51] 
Pascotini MET, Flores DAE, Kegler MA, et al. Brain-derived neurotrophic factor levels are lower in chronic stroke patients: A relation with manganese-dependent superoxide dismutase ALA16VAL single nucleotide polymorphism through tumor necrosis factor-α and caspases pathways. J Stroke Cerebrovasc Dis  2018; 27(11): 3020-9.
[http://dx.doi.org/10.1016/j.jstrokecerebrovasdis.2018.06.032] [PMID:  30150066] 
[52] 
Ansari MA, Scheff SW. Oxidative stress in the progression of Alzheimer disease in the frontal cortex. J Neuropathol Exp Neurol  2010; 69(2): 155-67.
[http://dx.doi.org/10.1097/NEN.0b013e3181cb5af4] [PMID:  20084018] 
[53] 
Kizer JR, Benkeser D, Arnold AM, et al. Advanced glycation/glycoxidation endproduct carboxymethyl-lysine and incidence of coronary heart disease and stroke in older adults. Atherosclerosis  2014; 235(1): 116-21.
[http://dx.doi.org/10.1016/j.atherosclerosis.2014.04.013] [PMID:  24825341] 
[54] 
Kjær LK, Cejvanovic V, Henriksen T, et al. Cardiovascular and all-cause mortality risk associated with urinary excretion of 8-oxoGuo, a biomarker for RNA oxidation, in patients with type 2 diabetes: A prospective cohort study. Diabetes Care  2017; 40(12): 1771-8.
[http://dx.doi.org/10.2337/dc17-1150] [PMID:  29061564] 
[55] 
Kurian P, Obisesan TO, Craddock TJA. Oxidative species-induced excitonic transport in tubulin aromatic networks: Potential implications for neurodegenerative disease. J Photochem Photobiol B  2017; 175: 109-24.
[http://dx.doi.org/10.1016/j.jphotobiol.2017.08.033] [PMID:  28865316] 
[56] 
Ramírez-Expósito MJ, Urbano-Polo N, Dueñas B, et al. Redox status in the sentinel lymph node of women with breast cancer. Ups J Med Sci  2017; 122(4): 207-16.
[http://dx.doi.org/10.1080/03009734.2017.1403522] [PMID:  29264992] 
[57] 
Shimamura N, Naraoka M, Matsuda N, et al. Intra-arterial oxidative stress correlates negatively with cognitive function and positively with postoperative ischemic lesions in carotid artery stenosis stenting. J Neurointerv Surg  2018; 10(5): 440-5.
[http://dx.doi.org/10.1136/neurintsurg-2017-013465] [PMID:  29184044] 
[58] 
Asghari G, Mirmiran P, Hosseni-Esfahani F, Nazeri P, Mehran M, Azizi F. Dietary quality among Tehranian adults in relation to lipid profile: findings from the Tehran Lipid and Glucose Study. J Health Popul Nutr  2013; 31(1): 37-48.
[http://dx.doi.org/10.3329/jhpn.v31i1.14747] [PMID:  23617203] 
[59] 
Nicklas TA, O’Neil CE, Fulgoni VL III. Diet quality is inversely related to cardiovascular risk factors in adults. J Nutr  2012; 142(12): 2112-8.
[http://dx.doi.org/10.3945/jn.112.164889] [PMID:  23077187] 
[60] 
Liu Z, Ren Z, Zhang J, et al. Role of ROS and nutritional antioxidants in human diseases. Front Physiol  2018; 9: 477.
[http://dx.doi.org/10.3389/fphys.2018.00477] [PMID:  29867535] 
[61] 
Xie Z, Lin H, Fang R, Shen W, Li S, Chen B. Effects of a fruit-vegetable dietary pattern on oxidative stress and genetic damage in coke oven workers: a cross-sectional study. Environ Health  2015; 14: 40.
[http://dx.doi.org/10.1186/s12940-015-0028-5] [PMID:  25943758] 
[62] 
WHO Fruit and vegetable promotion initiative-report of the meeting. Geneva: World Health Organization 2003.
[63] 
 Brasil Pesquisa Nacional de Saúde (PNS 2013): Percepção de
estado de saúde, estilo de vida e doenças crônicas Ministério da
	Saúde (MS) 2014.
[64] 
Olaya B, Moneta MV, Lara E, et al. Fruit and vegetable consumption and potential moderators associated with all-cause mortality in a representative sample of spanish older adults. Nutrients  2019; 11(8): 1794.
[http://dx.doi.org/10.3390/nu11081794] [PMID:  31382535] 
[65] 
Cai Q, Shu XO, Wen W, et al. Genetic polymorphism in the manganese superoxide dismutase gene, antioxidant intake, and breast cancer risk: results from the Shanghai Breast Cancer Study. Breast Cancer Res  2004; 6(6): R647-55.
[http://dx.doi.org/10.1186/bcr929] [PMID:  15535847] 
[66] 
Ibrahim HR, Hoq MI, Aoki T. Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding. Int J Biol Macromol  2007; 41(5): 631-40.
[http://dx.doi.org/10.1016/j.ijbiomac.2007.08.005] [PMID:  17919719] 
[67] 
Abeyrathne EDNS, Huang X, Ahn DU. Antioxidant, angiotensin-converting enzyme inhibitory activity and other functional properties of egg white proteins and their derived peptides - A review. Poult Sci  2018; 97(4): 1462-8.
[http://dx.doi.org/10.3382/ps/pex399] [PMID:  29340654] 
[68] 
Herrmann W, Obeid R. Homocysteine: A biomarker in neurodegenerative diseases. Clin Chem Lab Med  2011; 49(3): 435-41.
[PMID:  21388339] 
[69] 
Ko GJ, Kalantar-Zadeh K, Goldstein-Fuchs J, Rhee CM. Dietary approaches in the management of diabetic patients with kidney disease. Nutrients  2017; 9(8)E824
[http://dx.doi.org/10.3390/nu9080824] [PMID:  28758978] 
[70] 
Korai M, Kitazato KT, Tada Y, et al. Hyperhomocysteinemia induced by excessive methionine intake promotes rupture of cerebral aneurysms in ovariectomized rats. J Neuroinflammation  2016; 13(1): 165.
[http://dx.doi.org/10.1186/s12974-016-0634-3] [PMID:  27349749] 
[71] 
Mirmiran P, Bahadoran Z, Ghasemi A, Azizi F. Contribution of dietary amino acids composition to incidence of cardiovascular outcomes: A prospective population-based study. Nutr Metab Cardiovasc Dis  2017; 27(7): 633-41.
[http://dx.doi.org/10.1016/j.numecd.2017.05.003] [PMID:  28684082] 
[72] 
Montano MA, da Cruz IB, Duarte MM, et al. Inflammatory cytokines in vitro production are associated with Ala16Val superoxide dismutase gene polymorphism of peripheral blood mononuclear cells. Cytokine  2012; 60(1): 30-3.
[http://dx.doi.org/10.1016/j.cyto.2012.05.022] [PMID:  22688013] 
[73] 
Rashidipour-Fard N, Karimi M, Saraf-Bank S, Baghaei MH, Haghighatdoost F, Azadbakht L. Healthy eating index and cardiovascular risk factors among Iranian elderly individuals. ARYA Atheroscler  2017; 13(2): 56-65.
[PMID:  29026411] 

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DOI https://dx.doi.org/10.2174/1874609814666210526115150	Print ISSN 1874-6098
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Current Aging Science

Association of Val16Ala Polymorphism of Manganese Superoxide Dismutase (MnSOD) with Food Intake and Cardiometabolic Risk Factors in the Elderly in Primary Care in Porto Alegre

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