Title:In-silico Studies and Wet-Lab Validation of Camptothecin Derivatives for Anti-Cancer Activity Against Liver (HepG2) and Lung (A549) Cancer Cell Lines
Volume: 21
Issue: 10
Author(s): Komal Kalani, Dharmendra K. Yadav, Sarfaraz Alam, Feroz Khan, Mahendra P. Kashyap, Santosh K. Srivastava*Aditya B. Pant
Affiliation:
- Medicinal Chemistry Department, CSIR-CIMAP, Lucknow-226015 (U.P.) ,India
Keywords:
QSAR, ADME/T, Bioavailability, Docking, Camptothecin derivatives, Anticancer, Cytotoxic.
Abstract:
Background
: In the present study, we have explored the utility of QSAR modelling, in
silico ADMET, docking, chemical semi-synthesis, and in vitro evaluation studies for the identification
of active camptothecin (CPT) derivatives against cancer-targeting human liver (HepG2) and
lung (A549) cancer cell lines.
Methods: Two QSAR models were developed as screenings tools using the multiple linear regression
(MLR) method followed by ADMET and docking studies. The regression coefficient (r
2) and
cross-validation regression coefficients (rCV
2T) of the QSAR model for the HepG2 cell line was
0.95 and 0.90, respectively, and for the A549 cell line, it was 0.93 and 0.81, respectively.
Results: In silico studies show that CPT derivatives (CPT-1 and CPT-6) possess drug-like properties.
Docking performed on DNA Topoisomerase-I showed significant binding affinity. Finally, predicted
active derivatives were chemically semi synthesized, spectroscopically characterized, and
evaluated in-vitro for cytotoxic/anticancer activity against HepG2 and A549 cell lines.
Conclusion: The experimental results are consistent with the predicted results. These findings may
be of immense importance in the anticancer drug development from an inexpensive and widely
available natural product, camptothecin.
