Title:Protective Effect of the Combination of Wild-Type Genotypes (G/G and G/G) of CCR2-64V and SDF1-3A' Genes in Serodiscordant Couples in Yaounde-Cameroon
Volume: 19
Issue: 4
Author(s): Céline N. Nkenfou*, Constantin Tchakounté, Carine Nguefeu Nkenfou-Tchinda, Marie Nicole Ngoufack, Laeticia Grace Yatchou, Elise Elong, Rachel kamgaing, Aline Tiga, Georgia Ambada, Jules Roger Kuiate and Alexis Ndjolo
Affiliation:
- Chantal Biya International Reference Centre, (CBIRC), Yaounde,Cameroon
Keywords:
HIV, serodiscordant couple, seroconcordant couple, polymorphism associated with HIV/Aids related genes, protection,
susceptibility.
Abstract:
Background: There is growing evidence that polymorphisms in chemokine and
chemokine receptor genes influence susceptibility to HIV infection and disease progression. However,
not much is documented about the influence of these polymorphisms in HIV serodiscordant
couples in Cameroon.
Objective: The objective of this study therefore was to determine the prevalence and the effect of
the polymorphisms of CCR5-Δ32, CCR5 promoter 59029 A/G, CCR2-64I and SDF1-3′A gene in
HIV serodiscordant couples in comparison to HIV negative seroconcordant and HIV positive seroconcordant
couples in Yaoundé-Cameroon.
Methods: A total of 96 couples were recruited from five hospitals, of which 60 couples were HIV
serodiscordant (test group), 18 HIV negative seroconcordant and 18 HIV positive seroconcordant
couples were used as controls. Their genotypes for CCR5-Δ32, CCR5 promoter, CCR2 and SDF1
were analyzed using polymerase chain reaction (PCR) and restriction fragment length polymorphism.
Results: The allelic frequencies of these genes in the studied population were: 0%, 26.30%,
15.30% and 1.62% respectively for CCR5-Δ32, CCR5 promoter, CCR2 and SDF1. The frequency
of the combination of CCR5 promoter and SDF1- (A/A+ G/G) wild-type genotype was higher in
HIV-infected partners (82.92%) compared to uninfected partners (56.1%) in HIV serodiscordant
couples (p= 0.0001). The combination of wild-type CCR2 and SDF1 genotypes (G/G + G/G) was
higher among uninfected partners (80.48%) in HIV serodiscordant couples compared to the infected
partners (60.97) (p= 0.005).
Conclusion: HIV negative partner protection against HIV/AIDS infection may be attributed to the
combination of wild-type genotypes (G/G and G/G) of CCR2 and SDF1 genes in HIV serodiscordant
couples.
