![conference banner](/images/conference-banner.jpeg)
Abstract
Background: Cyclodextrins (CDs) are commonly used host molecules of inclusion complex. However, due to the lack of a sensitive determination method, the absorption process of CDs remains unclear.
Objective: In this study, an oleuropein (OL) inclusion complex employing hydroxylpropyl-betacyclodextrin (HP-beta-CD) as host molecules was prepared and the formation of inclusion complex was ascertained by FT-IR and DSC. Spectrophotometry was established for the determination of HP-beta-CD, based on the fact that the absorbance of phenolphthalein (PP) decreased in the presence of HP-beta-CD.
Methods: The assay conditions were optimized to augment the method sensitivity. Molecular docking was employed to verify the strong interaction between PP and HP-beta-CD. The permeation process of free HP-beta-CD, HP-beta-CD of OL inclusion complex, free OL, and OL in the inclusion complex, was examined using an in vitro mouse small intestine model.
Results: Though HP-beta-CD possessed a hydrophilic outside shell, it could permeate through the mouse small intestine quickly with a cumulative permeating amount of over 90% in 2 h. Free HPbeta- CD, the host molecule HP-beta-CD, and guest molecule OL of the inclusion complex exhibited consistent permeating profiles across the mouse small intestine.
Conclusion: The approach for the determination of HP-beta-CD was accurate and precise (%RSD=2.98).
Keywords: Hydroxylpropyl-beta-cyclodextrin, oleuropein, inclusion complex, phenolphthalein, spectrophotometry, intestinal permeation.
[http://dx.doi.org/10.1007/s10847-020-01011-z]
[http://dx.doi.org/10.1016/j.molstruc.2020.128161]
[http://dx.doi.org/10.1016/j.foodhyd.2020.105882]
[http://dx.doi.org/10.1208/s12249-020-01724-x] [PMID: 32632576]
[http://dx.doi.org/10.1007/s10973-020-09549-3]
[http://dx.doi.org/10.1111/cbdd.13664] [PMID: 32691965]
[http://dx.doi.org/10.1016/j.carbpol.2020.116267] [PMID: 32475557]
[http://dx.doi.org/10.1016/j.ijpharm.2020.119301] [PMID: 32268184]
[http://dx.doi.org/10.1016/j.ultsonch.2020.104997] [PMID: 32058914]
[http://dx.doi.org/10.1016/j.jddst.2020.101742]
[http://dx.doi.org/10.1016/j.molliq.2020.113257]
[http://dx.doi.org/10.1016/j.molliq.2020.113118]
[http://dx.doi.org/10.1016/j.jhazmat.2020.122776] [PMID: 32334288]
[http://dx.doi.org/10.1016/j.microc.2020.104994]
[http://dx.doi.org/10.1039/D0NJ01516D]
[http://dx.doi.org/10.1016/j.chroma.2020.461128] [PMID: 32331779]
[http://dx.doi.org/10.1039/C2AN36003A] [PMID: 23139926]
[http://dx.doi.org/10.3390/antiox9020149] [PMID: 32050687]
[http://dx.doi.org/10.1016/j.carbpol.2019.115166] [PMID: 31472864]
[http://dx.doi.org/10.1007/s10847-005-0245-6]
[http://dx.doi.org/10.1016/j.carbpol.2018.07.019] [PMID: 30143174]
[http://dx.doi.org/10.1016/j.foodhyd.2019.01.011]
[http://dx.doi.org/10.1016/j.foodhyd.2019.105202]
[http://dx.doi.org/10.1021/jo980613i]
[http://dx.doi.org/10.1016/j.foodchem.2019.02.021] [PMID: 30827631]
[http://dx.doi.org/10.2174/1381612822666160720163656] [PMID: 27510485]
[http://dx.doi.org/10.1021/acs.jafc.9b01355] [PMID: 30929431]
[http://dx.doi.org/10.1016/j.jpba.2018.04.028] [PMID: 29702390]
[http://dx.doi.org/10.1089/jmf.2016.3725] [PMID: 27982756]
[http://dx.doi.org/10.1134/S1068162020050246]
[http://dx.doi.org/10.1248/cpb.40.1252] [PMID: 1394642]
[http://dx.doi.org/10.1080/03639045.2016.1264412] [PMID: 27892724]
[http://dx.doi.org/10.1002/cpt.1943]
[http://dx.doi.org/10.3390/nu12030669] [PMID: 32121413]
[http://dx.doi.org/10.1016/j.ijpharm.2015.10.049] [PMID: 26498369]
[http://dx.doi.org/10.1073/pnas.0914309107] [PMID: 20212119]
[http://dx.doi.org/10.1371/journal.pone.0084856] [PMID: 24416301]
![Wayfinder Image](/images/wayfinder.jpg)