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当代阿耳茨海默病研究

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ISSN (Print): 1567-2050
ISSN (Online): 1875-5828

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Research Article

体内/体外EPR研究5xFAD小鼠阿尔茨海默病模型的脑氧化还原状态和血脑屏障完整性

卷 18, 期 1, 2021

发表于: 24 March, 2021

页: [25 - 34] 页: 10

弟呕挨: 10.2174/1567205018666210324121156

价格: $65

Open Access Journals Promotions 2
摘要

背景：阿尔茨海默病(AD)是最常见的神经退行性疾病，其特征是认知能力下降和完全脑萎缩。尽管付出了大量的科学努力，阿尔茨海默病神经退行性变的病理机制目前尚不清楚。在大多数研究中，淀粉样β肽被认为是AD的关键病理改变。然而，许多Aβ靶向治疗在临床试验中都失败了。这意味着需要将研究重点从Aβ转移到该病的其他病理特征上。 目的：本研究的目的是通过电子顺磁共振(EPR)光谱的应用，研究AD病理过程中线粒体功能障碍、氧化应激和血脑屏障(BBB)破坏之间的相互作用。 方法：采用两种具有不同细胞膜和血脑屏障通透性的自旋探针(氨基氧自由基)进行体内和体外EPR波谱分析，评估5xFAD小鼠模型的血脑屏障完整性和脑组织氧化还原状态。体内自旋探针还原衰减采用双室药代动力学模型进行分析。采用15K电子顺磁共振(EPR)技术检测脑金属含量。 结果：本研究揭示了5xFAD模型中大脑氧化还原状态的改变、血脑屏障的破坏、ROS介导的线粒体铁硫簇损伤以及MnSOD的上调。 结论:EPR自旋探针在5xFAD神经元组织氧化还原状态和血脑屏障完整性方面具有良好的体内报告功能，表明EPR光谱在神经退行性疾病的临床前研究中具有重要的应用价值。

关键词: 阿尔茨海默病，血脑屏障，EPR，线粒体，旋转探针，5xFAD

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