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Current Bioactive Compounds


ISSN (Print): 1573-4072
ISSN (Online): 1875-6646

Research Article

Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of Alphonsea madraspatana

Author(s): Amita Sahu, Sudhanshu Shekhar Swain, Goutam Ghosh, Deepak Pradhan, Dipak Kumar Sahu, Prativa Biswasroy and Goutam Rath*

Volume 17, Issue 10, 2021

Published on: 22 February, 2021

Article ID: e190721191719 Pages: 10

DOI: 10.2174/1573407217666210223092824

Price: $65


Background: Literature evidence as well as traditional uses of genus Alphonsea reveal significant antimicrobial and anti-oxidant activitiesencouraging to consider A. madraspatana to have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from the extract of A. madraspatana leaves (AML) and to evaluate the effect against bacteria.

Methods: BAgNPs were synthesized by the optimized reaction. The Synthesized nanoparticles were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized BAgNPs was evaluated against E. coli by comparing with positive controls.

Results: Results of the optimization process indicate nanoscale BAgNPs were produced at 45°C for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed nanosilver (28± 2 mm). The developed BAgNPs show comparable biofilm inhibition (86.50%) as compared to marketed nanosilver (88.10%) and Ciprofloxacin (83.10%).

Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions, which successfully generate nano-template of silver with better antibacterial response against E. coli.

Keywords: A. madraspatana, silver nanoparticles, optimization, E. coli, antibiofilm activity, antimicrobial resistance, AML.

Graphical Abstract
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