Title:Optimization and Characterization of Biogenic Silver Nanoparticles Synthesized by Leaves Extract of Alphonsea madraspatana
Volume: 17
Issue: 10
Author(s): Amita Sahu, Sudhanshu Shekhar Swain, Goutam Ghosh, Deepak Pradhan, Dipak Kumar Sahu, Prativa Biswasroy and Goutam Rath*
Affiliation:
- School of Pharmaceutical Sciences, Department of Pharmacognosy, Siksha ‘O’ Anusandhan Deemed to be University Bhubaneswar, Odisha,India
Keywords:
A. madraspatana, silver nanoparticles, optimization, E. coli, antibiofilm activity, antimicrobial resistance, AML.
Abstract:
Background: Literature evidence as well as traditional uses of genus Alphonsea reveal
significant antimicrobial and anti-oxidant activitiesencouraging to consider A. madraspatana to
have potent antimicrobials, there by offering potential adjuncts to synthesize improved antimicrobial
Silver nanoparticles (AgNPs). The objective of the present exposition is to optimize reaction
parameters to synthesize antimicrobial Biogenic Silver nanoparticles (BAgNPs) from the extract of
A. madraspatana leaves (AML) and to evaluate the effect against bacteria.
Methods: BAgNPs were synthesized by the optimized reaction. The Synthesized nanoparticles
were characterized by UV, IR, ICP-MS and XRD analysis. The antibacterial potency of optimized
BAgNPs was evaluated against E. coli by comparing with positive controls.
Results: Results of the optimization process indicate nanoscale BAgNPs were produced at 45°C
for 120 min at pH 8 with 1:5 volume ratio of AgNO3 and extract. Optimized BAgNPs exhibits relatively
higher antimicrobial activity (31±1mm) compared to Ciprofloxacin (27±1mm) and marketed
nanosilver (28± 2 mm). The developed BAgNPs show comparable biofilm inhibition (86.50%) as
compared to marketed nanosilver (88.10%) and Ciprofloxacin (83.10%).
Conclusion: Experimental evidence suggests methanolic extract of AML under predefined conditions,
which successfully generate nano-template of silver with better antibacterial response against
E. coli.
