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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Determination of Total Arsenic, Soluble Arsenic, Total Mercury and Soluble Mercury for a Realgar and Cinnabar-containing Traditional Chinese Medicine Compound Niuhuang Xiaoyan Capsule by Semi-bionic Extraction- ICP-MS

Author(s): Bo-Yang Xu, Si-Qi Zhu, Xian-Xin Cheng, Yu Xu* and Ji-Wu Liu*

Volume 18, Issue 2, 2022

Published on: 11 February, 2021

Page: [193 - 198] Pages: 6

DOI: 10.2174/1573412917666210211122821

Price: $65

Abstract

Background: Compound Niuhuang Xiaoyan capsule is a realgar and cinnabar-containing traditional Chinese medicine, the contents of total arsenic and total mercury are found over regulation defined limits. However, only small amounts of arsenic in realgar and mercury in cinnabar are dissolved in the gastric juice, so the determination of soluble arsenic and soluble mercury is more significant than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule.

Objective: To study the contents of total arsenic, total mercury, soluble arsenic and soluble mercury in Compound Niuhuang Xiaoyan capsule.

Methods: Microwave digestion and semi-bionic extraction were used to pretreat the samples of Compound Niuhuang Xiaoyan capsule, and the inductively coupled plasma mass spectrometry method was established to determine the contents of total arsenic, total mercury, soluble arsenic and soluble mercury in Compound Niuhuang Xiaoyan capsule. The accuracy of the established method was further evaluated by using a certified standard reference material prepared from dried citrus leaves (GBW10020(GSB-11)).

Results: The spiked recoveries were within 95-105 %. The R.S.D% of repeatability (N=6) and precision (N = 6) were below 5.0 %. The correlation coefficients of linear (R) for arsenic and mercury were both above 0.998. The limits of quantification (LOQ) were below 0.1 μg/L. The contents of As and Hg were defined in the dried citrus leaves and very near to the standard values provided by the manufacturer. The established method was applied for the analysis of three batches of Compound Niuhuang Xiaoyan capsule produced by three different manufacturers successfully. Insufficient realgar or cinnabar was formulated in some Compound Niuhuang Xiaoyan capsule samples.

Conclusion: The analysis showed that the contents of soluble arsenic and soluble mercury in artificial gastric juice were significantly less than that of total arsenic and total mercury in Compound Niuhuang Xiaoyan capsule. The results provided a reference for further study on the toxicology and pharmacokinetics of Compound Niuhuang Xiaoyan capsule.

Keywords: Inductively coupled plasma mass spectrometry, Compound Niuhuang Xiaoyan capsule, semi-bionic extraction, realgar and cinnabar, total arsenic and mercury, soluble arsenic and mercury.

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[1]
Chinese Pharmacopeia Commission; Beijing China Medical Science Press, 2015, Vol. I, pp. 1211-1212.
[2]
Sanchez, T.R.; Perzanowski, M.; Graziano, J.H. Inorganic arsenic and respiratory health, from early life exposure to sex-specific effects: A systematic review. Environ. Res., 2016, 147, 537-555.
[http://dx.doi.org/10.1016/j.envres.2016.02.009] [PMID: 26891939]
[3]
Liu, X.J.; Liu, W.J.; Lin, A.J.; Liu, Y.X. Survey of arsenic concentrations in Chinese herbal medicines (CHMs) and preliminary risk assessment of As in CHMs on human health. Huan Jing Ke Xue, 2010, 31(12), 3036-3042.
[PMID: 21360896]
[4]
Koch, I.; Sylvester, S.; Lai, V.W.; Owen, A.; Reimer, K.J.; Cullen, W.R. Bioaccessibility and excretion of arsenic in Niu Huang Jie Du Pian pills. Toxicol. Appl. Pharmacol., 2007, 222(3), 357-364.
[http://dx.doi.org/10.1016/j.taap.2006.12.005] [PMID: 17239412]
[5]
Zhang, Y.N.; Li, H.Y.; Zheng, L.L.; Ling, Q.F.; Lei, L.B. Atomic fluorescence determination of cinnabar-containing traditional Chinese medicine preparations of soluble mercury salts. Chin. J. Health Lab. Technol., 2010, 20(4), 758-760.
[6]
Liang, A.; Wang, J.; Xue, B.; Li, C.; Liu, T.; Zhao, Y.; Cao, C.; Yi, Y. Study on hepatoxicity and nephrotoxicity of cinnabar in rats. Zhongguo Zhongyao Zazhi, 2009, 34(3), 312-318.
[PMID: 19445157]
[7]
Tang, Y.S.; Wang, N.S. Effects of components in Angong Niuhuang Pulvis on solubility of arsenic in realgar. J. Mod. Food Pharm., 2006, 16(3), 4-7.
[8]
Wu, X.H.; Sun, D.H.; Zhuang, Z.X.; Wang, X.L.; Gong, H.F.; Hong, J.X.; Frank, S.C. Analysis and leaching characteristics of mercury and arsenic in Chinese medicinal materials. Anal. Chim. Acta, 2002, 453(2), 311-323.
[http://dx.doi.org/10.1016/S0003-2670(01)01442-8]
[9]
Huang, Q.C.; Wei, Y.H.; Wu, Y.Z. Study on the harmful effect of arsenic pollution on human health. Stud. Trace. Elem. Health., 2009, 26(4), 65-67.
[10]
Ma, Y.J.; Li, J.; Liu, X.; Bi, J.Q. Biomimetic extraction of ICP - OES for determination of acid-soluble heavy metals and total heavy metals in four kinds of Chinese patent medicines containing realgar. Chin. Arch. Tradit. Chin. Med., 2014, 32(3), 647-649.
[11]
Jin, P.; Liang, X.; Xia, L.; Jahouh, F.; Wang, R.; Kuang, Y.; Hu, X. Determination of 20 trace elements and arsenic species for a realgar-containing traditional Chinese medicine Niuhuang Jiedu tablets by direct inductively coupled plasma-mass spectrometry and high performance liquid chromatography-inductively coupled plasma-mass spectrometry. J. Trace Elem. Med. Biol., 2016, 33, 73-80.
[http://dx.doi.org/10.1016/j.jtemb.2015.09.006] [PMID: 26653746]
[12]
Avula, B.; Wang, Y-H.; Smillie, T.J.; Duzgoren-Aydin, N.S.; Khan, I.A. Quantitative determination of multiple elements in botanicals and dietary supplements using ICP-MS. J. Agric. Food Chem., 2010, 58(16), 8887-8894.
[http://dx.doi.org/10.1021/jf101598g] [PMID: 20681579]
[13]
Dart, R.C. Medical Toxicology, 3rd ed; Lippincott Williams & Wilkins: New York, 2004.
[14]
http://www.satcm.gov.cn/fajiansi/gongzuodongtai/2018-03-24/2394.html2001 Available from
[15]
Xu, Y.Y.; Wang, Q.; Fu, X.P.; Song, Y.; Mu, Y.C.; Meng, D. Research progress of arsenic exposure assessment in food. Zhongguo Nongxue Tongbao, 2014, 30(34), 187-192.
[16]
Jiao, Y.; Chen, T. Detoxification therapy for a case of chronic arsenic poisoning caused by long-term taking Niuhuang Jiedu Pian. J. Tradit. Chin. Med., 2007, 48(8), 724-725.
[17]
Pagano, G.; Thomas, P.J.; Di Nunzio, A.; Trifuoggi, M. Human exposures to rare earth elements: Present knowledge and research prospects. Environ. Res., 2019, 171, 493-500.
[http://dx.doi.org/10.1016/j.envres.2019.02.004] [PMID: 30743241]
[18]
Bai, G.Y.; Wei, C.; Ouyang, L.; Xie, Q.; Liu, Y.Q.; Wang, J.; Wang, J.Y. Determination of 5 heavy metals in Chinese traditional medicines and extraction liquid containing mineral materials. Guangpuxue Yu Guangpu Fenxi, 2011, 31(1), 256-259.
[PMID: 21428100]
[19]
Jin, P.F.; Liang, X.L.; Xia, L.F.; Guang, Y.M.; Hu, X.; Zhou, D. Study on total contents of 20 trace elements in Niuhuang Jiedu tablets and their extract rates in water and gastrointestinal fluid by ICP-MS. Yaowu Fenxi Zazhi, 2014, 34(6), 985-991.
[20]
Yu, Y.J.; Wang, F.F.; Fang, J.D.; Sun, P. Advance in research on environmental arsenic pollution to human health. Zhongguo Nongxue Tongbao, 2007, 24(3), 181-183.
[21]
Zhang, W.; Liu, X.L.; Luo, G.F.; Liu, Y.R.; Yuan, L.; Xiao, R.N.; Zhang, Y.Y. Comparative study of total and soluble arsenic, mercury in the Tsothel and other precious Tibetan medicine. Yaowu Fenxi Zazhi, 2016, 36(4), 721-726.

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