Title:Effective Inhibition of Mitochondrial Metabolism by Cryptotanshinone in
MDA-MB231 cells: A Proteomic Analysis
Volume: 19
Issue: 1
Author(s): Jiefeng Zhou, Qingcao Li, Haoran Wu, Shin-Han Tsai and Yu-Ting Yeh*
Affiliation:
- Graduate Institute of Biomedical Informatics, College of Medical Science and Technology, Taipei Medical University,-
Taipei City, Taiwan
- Information Technology Office, Shuang Ho Hospital, Taipei Medical University, New Taipei City, Taiwan
Keywords:
Cryptotanshinone, proteomic, breast cancer, MDA-MB-231, mitochondrial, TNBC.
Abstract: Background: Triple-negative breast cancer (TNBC) is a subtype of invasive cancer in
breast with the symptoms of unfavourable prognosis and limited targeted treatment options. Evidence
of changes in the metabolic status of TNBC, characterised by increased glycolysis, mitochondrial
oxidative phosphorylation, as well as production and utilization of tricarboxylic acid cycle intermediates.
Objective: The objective of this study is to investigate the proteins altered in cryptotanshinone treated
MDA-MB-231 cells and explore the key pathways and specific molecular markers involved in
cryptotanshinone treatment.
Methods: We use unlabeled quantitative proteomics to gain insight into the anticancer mechanism
of cryptotanshinone on MDA-MB231 triple negative breast cancer cells. And flow cytometry was
used to detect apoptosis and changes in cell mitochondrial membrane potential.
Results: We show that inhibiting the expression of electron transport chain complex proteins, also
inhibits mitochondrial oxidative phosphorylation. Additionally, down-regulation of the ribosime
biogenesis pathway was found to inhibit cell metabolism.
Conclusion: In summary, results show that cryptotanshinone can trigger rapid and irreversible
apoptosis in MDA-MB-231 cells through effectively inhibiting cell metabolism.