Title:Role of DACH1 on Proliferation, Invasion, and Apoptosis in Human Lung Adenocarcinoma Cells
Volume: 21
Issue: 9
关键词:
肺腺癌，DACH1，增殖，侵袭，凋亡，RT-PCR。
摘要:
Objective: To investigate DACH1 protein expression in lung cancer tissue
and matched paracancerous tissue and explore its effect on proliferation, invasion, and
apoptosis in human lung adenocarcinoma cells (HLACs).
Methods: Tumor tissue and matched paracancerous tissue were collected from 46
patients with pathologically diagnosed lung cancer. RT-PCR was performed to detect
DACH1 mRNA expression and immunohistochemistry to measured DACH1 protein
expression. To determine the effect of DACH1 on lung cancer behavior, small interfering
RNA (siRNA) was used to silence DACH1 expression in A549 cells. The impact on the
proliferation of tumor cells was then observed by MTT assay, changes in the invasion of
tumor cells were identified using transwell chamber assay, and the effects on apoptosis
in the cell line were detected using flow cytometry.
Results: The expression of DACH1 mRNA and DACH1 protein was significantly
decreased in lung cancer tissue versus matched paracancerous control tissue. The
silencing of DACH1 expression in A549 cells significantly enhanced cell proliferation,
significantly increased cell invasion, and significantly reduced spontaneous apoptosis.
Conclusion: DACH1 is downregulated in lung adenocarcinoma tissue. In vitro
assessment shows that DACH1 functions as a tumor suppressor, suggesting its
potential use as a new target for lung cancer treatment.
