Title:A Gas Chromatography Flame Ionization Detector Method for Rapid Simultaneous Separation and Determination of Six Active Ingredients of Anticold Drug
Volume: 18
Issue: 1
Author(s): Fatang Yang, Xiaoyun Duan, Zhen Wang and Yuming Dong*
Affiliation:
- School of Pharmacy, Lanzhou University, Lanzhou, 730000,China
Keywords:
Gas chromatography, flame ionization detector, active ingredients, anti-cold drug, sensitivity, precision.
Abstract:
Aims: To establish a rapid and simultaneous determination of multiple effective ingredients
in anti-cold drugs.
Background: Anti-cold drugs are stock medicines at home, and most anti-cold formulations are
compound preparations. Although the active ingredients of compound preparations have significant
effects on the treatment of colds, the excessive dosage or long-term use can produce a series
of adverse reactions, including dependence, liver and kidney function damage, digestive system
reaction, blood system damage. Now, there are many mature methods for analyzing the active ingredients
of anti-cold drugs. However, these methods may have shortcomings, such as a long
analysis time or a small number of analysis components.
Objective: Establish a gas chromatography-flame ionization detector method for the simultaneous
determination of six active ingredients, including acetaminophen, dextromethorphan hydrobromide,
pseudoephedrine hydrochloride, chlorpheniramine maleate, diphenhydramine hydrochloride,
and caffeine in anti-cold drugs.
Methods: After the standard was accurately weighed, dissolved in ethanol, filtered by 0.22 μm
membrane and ultrasonically degassed, the gas chromatograph was used for detection. After the
actual sample was removed from the coating, ground and crushed, accurately weighed, dissolved
in ethanol, filtered by 0.22 μm membrane and ultrasonically degassed, the gas chromatograph was
used for detection.
Results: The six components can be completely separated within 7.0min. This method has good
sensitivity, precision, accuracy and recovery rate. Under the optimum testing conditions, the limit
of detection was 0.360-2.50μg/mL, the limit of quantification was 1.20-8.30μg/mL. The calibration
curves showed good linearity (R2≥0.9932) over the investigated concentration range between
1.20 and 400μg/mL. The recoveries were 89.2% to 109.2%. The RSD of intra-day precision was
less than 1.0%. The RSD of inter-day precision was less than 3.2%. The established method was
used to determine the ingredients of three anti-cold drugs on the market, and the results showed
that the method can accurately determine the ingredients.
Conclusion: The method can quickly and simultaneously determine multiple active ingredients in
anti-cold medicines. Compared with the published methods in literature, the proposed method has
the advantages of fast, the number of analysis components wide application range, convenience,
low cost, etc. It provides a reference method for quality control of active ingredients of anti-cold
drugs.
