Title:miR-375 Inhibits the Proliferation, Migration and Invasion of Esophageal Squamous Cell Carcinoma by Targeting XPR1
Volume: 21
Issue: 4
Author(s): Wenbin Wu, Yangmei Zhang, Xiaowu Li, Xiang Wang*Yuan Yuan*
Affiliation:
- Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009,China
- Department of Medical Oncology, Xuzhou Central Hospital, Xuzhou Medical University, Xuzhou, 221009,China
Keywords:
XPR1, miR-375, esophageal squamous cell carcinoma, proliferation, migration, invasion.
Abstract:
Objective: The purpose of this study was to explore the mechanism of the miR-375/XPR1
axis in esophageal squamous cell carcinoma (ESCC) and provide a new idea for targeted therapy
of ESCC.
Methods: Differentially expressed genes in GEO and TCGA databases were analyzed by bioinformatics.
The expression levels of miR-375 and XPR1 mRNA were detected by qRT-PCR. Protein
expression of XPR1 was detected by western blot. Bioinformatics analysis and dual luciferase assay
were conducted to confirm the target relationship between miR-375 and XPR1. The viability,
proliferation, migration and invasion of cells in each treatment group were detected by CCK-8,
colony formation, wound healing and Transwell assays.
Results: Significantly down-regulated miR-375 and remarkably up-regulated XPR1 were observed
in ESCC tissue and cells. Overexpression of miR-375 inhibited proliferation, invasion and migration
of ESCC cells, and greatly reduced the promoting effect of XPR1 overexpression on cell proliferation,
migration and invasion. Dual luciferase assay confirmed that miR-375 targeted and inhibited
XPR1 expression in ESCC.
Conclusion: These results demonstrate the regulatory role of the miR-375/XPR1 axis in ESCC
cells and provide a new potential target for the precise treatment of patients with ESCC.
