Title:Genome Editing with CRISPR-Cas9: A Budding Biological Contrivance for Colorectal Carcinoma Research and its Perspective in Molecular Medicine
Volume: 21
Issue: 6
Author(s): Suman K. Ray and Sukhes Mukherjee*
Affiliation:
- Department of Biochemistry, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh-462020,India
Keywords:
Genome editing, CRISPR/Cas9 technology, colorectal carcinoma, off-target impacts, Molecular
medicine, protein-based technology.
Abstract: Genome editing is an addition, deletion, or replacement of a gene to remove
or initiating explicit and preferred characters in the genome. Utilizing gene-editing tools
like CRISPR-Cas9 technology could be accomplished either by gene-based
methodology or protein-based technology that has been under scrutiny for the protracted
time wherein physical techniques, viral and non-viral strategies have been utilized
together. Transplanting ex vivo CRISPR edited cells empowers screening of single
guide RNAs with high-throughput and CRISPR based screening in organoids
transplantation to validate cancer cells including colorectal carcinoma in various phases
of its development and treatment.CRISPR knockout screens have recognized genes
that drive interest in colon cancer to develop hallmarks, especially in some cancer cell
lines with single guide RNA, to disclose drug resistance mechanisms. One advantage of
this method is to deal with CRISPR knockout genomic screening, which disrupts gene
expression, rather than the partial knockdown that is mostly done with RNA interference
and CRISPR/Cas technology. This technique is used to treat different forms of cancer
because of its proficient editing of the target gene, along with the CRISPR/Cas system.
Latest research has shown that the CRISPR/Cas gene-editing technique has
theoretically reformed the expression in colorectal carcinoma of long non-coding RNA.
For the next decade CRISPR/Cas9 technology will positively fuel the development of
more in vivo gene editing clinical trials in colon cancer and will have an enormous impact
on molecular medicine.
