Title:Scanning Electron Microscopy Analysis, Fourier Transform Infrared Spectrum Structure Profile Characterisation, and in vitro Biological Effect of Salvia officinalis L. Phytocompounds
Volume: 22
Issue: 10
Author(s): Issam Saidi, Fatma Guesmi*, Ramzi Amari, Sana Ncib and Najla Hfaiedh
Affiliation:
- Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, Gafsa 2112,Tunisia
Keywords:
Salvia officinalis, FT-IR, SEM, phenolic content, antioxidant activity, antibacterial effect.
Abstract:
Background: The aim of this research is to investigate the potential activity of Salvia officinalis
and various hair samples responsible for secretion of essential oil. In Tunisia, biological activity
of Salvia officinalis is poorly recorded. Salvia leaves contain various types of hairs (glandular and nonglandular).
Methods: The investigation of different trichomes was carried out by Scanning Electron Microscope
(SEM) apparatus. Antiradical potential was assessed using 2,2-Diphenyl-1-Picrylhydrazyl (DPPH) and
Ferric Reducing Antioxidant Power (FRAP) assay. Antimicrobial activity was analysed using disc diffusion
assay. The extracts of Salvia officinalis (SvOf), showed the following order of richness in phenolic
contents: methanolic (70.76 mg GAE/g DW), aqueous (43.76 mg GAE/g DW) and infusion (9.42
mg GAE/g DW). The methanolic fraction records the highest levels of flavonoids (77 mg QE/g DW)
compared with the aqueous extracts (33.19 mg EQ/g DW) and infusion (26.25 mg EQ/g DW). MESvOf
showed higher value of free radical scavenging activity towards DPPH free radical and ferric reducing
power.
Results: The results revealed that the main bioactive constituents in the methanolic fractions of sage
leaves generally exhibited higher antibacterial effects. Overall, sage phytocompounds constitute a
promising approach for the treatment of infectious diseases.
Discussion: Functional groups detected in S. officinalis by Fourier Transform Infrared Spectrophotometer
(FT-IR) were mainly phenols, saccharides, amine and Aromatic (Ar-H).
Conclusion: Antiradical and antibacterial activities of Salvia officinalis are mainly due to phenolic
content and other bioactive compounds. Non-glandular hairs are the most important trichomes in the
Salvia leaves.
