Title:Extraction and Evaluation of Anti-inflammatory and Analgesic Activity of Mimosa rubicaulis in Swiss Albino Rats
Volume: 19
Issue: 5
Author(s): Roshani Gurung, Sundar Adhikari, Niranjan Koirala and Kalpana Parajuli*
Affiliation:
- Department of Pharmaceutical Sciences, School of Health and Allied Sciences, Pokhara University, Pokhara-30, Kaski, 33700,Nepal
Keywords:
Analgesic activity, anti-inflammatory activity, carrageenan, hot plate method, tail immersion method, Mimosa rubicaulis.
Abstract: Background: Pain and inflammation are major health problems. The adverse effect of
synthetic drugs for the management of painful inflammatory conditions gives a strong motivation
for researchers to develop newer drugs with lesser side effects. Mimosa rubicaulis has been traditionally
used in rheumatism, fungal infection, wound, fever, etc.
Objective: The objective was to evaluate the anti-inflammatory and analgesic activity of ethanolic
stem extract of M. rubicaulis in Swiss albino rats.
Methods: The stem of M. rubicaulis was collected, subjected to double maceration using ethanol
as a solvent. Then, Swiss albino rats (n=72) were taken. An acute toxicity study was conducted using
different doses of the plant extract. The anti-inflammatory activity (100 mg/kg, 250 mg/kg)
was evaluated using the carrageenan-induced paw edema test, while, analgesic activity (100
mg/kg, 250 mg/kg) was evaluated using a hot plate and tail immersion method. The result was analyzed
using a one-way analysis of variance and post hoc analysis with the Tukey test.
Results: The acute toxicity study showed that the plant is safe for use in an animal model. The extract
showed anti-inflammatory activity by a reduction in paw edema volume by 25% and 48%.
Similarly, analgesic activity was shown by an increase in latency of paw licking and tail withdrawal
by 41%, and 56% (hot plate method) and 39% and 54% (Tail immersion method), p<0.05.
Conclusion: It can be concluded that the ethanolic extract of Mimosa rubicaulis is safe and possesses
both anti-inflammatory and analgesic activity.
