Investigation and Analytical Applications of the Reaction of N¹-methylnicotinamide and Active Methylene Containing Drugs

Title:Investigation and Analytical Applications of the Reaction of N¹-methylnicotinamide and Active Methylene Containing Drugs

Volume: 17 Issue: 10

Author(s): Heba A. Elsebaie, Mervat H. Elhamamsy, Khaled M. Elokely*Mohammed A. Eldawy

Affiliation:

• Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Tanta University, Tanta31527,Egypt

Keywords: Spectrofluorometric analysis, modafinil, budesonide, allicin, florfenicol, free energy calculations.

Abstract:

Background: The reaction between N¹-methylnicotinamide iodide (NMNI) and the active methylene group-containing compounds yields a fluorescent product, which can be used in the quantitative determination of certain drugs.

Objective: The objective is to develop, adapt, and validate a simple spectrofluorometric method for the quantitative analysis of modafinil 1, budesonide 2, allicin 3, and florfenicol 4.

Methods: Spectral analysis (elemental analysis, ¹H, ¹³C NMR, MS), computational methods (geometry optimization, Gibbs free energy and pK_a calculations) and spectrofluorometric approaches were conducted.

Result: A C4 cyclization pathway was confirmed for the formation of the fluorophore. The spectrofluorometric method showed good linearity (R²= 0.996-0.999) over concentrations of 1-50 ng/mL, 0.5-5 ng/mL, 60-150 pg/mL, and 1-15 ng/mL of the standard solutions of 1, 2, 3, and 4, respectively.

Conclusion: The proposed method is simple and suitable for quality control and assurance studies.

Cite this article as:

Elsebaie A. Heba, Elhamamsy H. Mervat , Elokely M. Khaled *, Eldawy A. Mohammed , Investigation and Analytical Applications of the Reaction of N¹-methylnicotinamide and Active Methylene Containing Drugs, Current Pharmaceutical Analysis 2021; 17 (10) . https://dx.doi.org/10.2174/1573412917999200922131030