![conference banner](/images/conference-banner.jpeg)
Abstract
Background: This study aimed to determine the effect and mechanism of Xiaoaiping (XAP) injection combined with S-1 in inhibiting the invasion and metastasis of human GC cells.
Methods: BGC-823 and MGC-803 cells were incubated in vitro, and the effects of treatment on the cytotoxicity and proliferation of BGC-823 and MGC-803 cells were evaluated by MTT assay. Cell adhesion tests and Transwell assays were used to detect the effects of Xiaoaiping injection combined with S-1 on the metastatic ability of BGC-823 and MGC-803 cells. The expression of VEGF, Metalloproteinases (MMPs) and proteins related to the Epithelial-Mesenchymal Transition (EMT) were detected by Western blotting. Meanwhile, a tumour model was established in nude mice, and the effect of XAP combined with S-1 on BGC-823 cells in vivo was studied.
Results: Compared with the single drug group, the combination of XAP with S-1 increased the inhibition rate (P<0.05). The adhesion test showed that the combination group significantly inhibited the adhesion of BGC-823 and MGC-803 cells (P<0.05). The combination of XAP with S-1 reduced the migration and invasion potential of human GC BGC-823 and MGC-803 cells. Western blotting showed that the expression of VEGF, MMP-9, Ncadherin and vimentin was decreased and E-cadherin expression was increased in the combination group compared with these expression values in either the XAP or S-1 alone group (P<0.05). In vivo, we found that XAP combined with S-1 had a significant inhibitory effect on the growth of tumours compared with XAP or S-1 alone. Immunohistochemistry showed that XAP combined with S-1 was able to enhance the levels of E-cadherin and downregulate N-cadherin and vimentin.
Conclusion: The combination of XAP with S-1 can enhance the inhibitory effect of a single drug on proliferation, invasion and metastasis. The mechanism may be related to the decrease in the expression of VEGF and MMP-9 proteins and the effect on EMT.
Keywords: Xiaoaiping (XAP) injection, S-1, invasion and metastasis, GC, MMP-9, VEGF, Epithelial-Mesenchymal Transition (EMT).
[http://dx.doi.org/10.1158/1055-9965.EPI-13-1057] [PMID: 24618998]
[PMID: 24494544]
[PMID: 26137702]
[http://dx.doi.org/10.1097/00001813-199607000-00010] [PMID: 8862723]
[PMID: 11106261]
[PMID: 3114201]
[PMID: 7689420]
[http://dx.doi.org/10.1007/s00280-015-2738-3] [PMID: 25862350]
[http://dx.doi.org/10.1186/s12885-018-4109-z] [PMID: 29439671]
[http://dx.doi.org/10.1007/s11655-016-2271-1] [PMID: 27921195]
[http://dx.doi.org/10.1016/j.biopha.2017.03.012] [PMID: 28320083]
[PMID: 22967439]
[http://dx.doi.org/10.3390/jcm7010001] [PMID: 29271928]
[http://dx.doi.org/10.1038/nrc.2017.118] [PMID: 29326430]
[PMID: 27617575]
[http://dx.doi.org/10.1038/s41586-018-0040-3] [PMID: 29670281]
[http://dx.doi.org/10.1186/s12885-018-4000-y] [PMID: 29361914]
[http://dx.doi.org/10.1186/s12957-016-1009-z] [PMID: 27716367]
[http://dx.doi.org/10.7150/thno.23209] [PMID: 29774078]
[http://dx.doi.org/10.1016/j.ajpath.2015.05.017] [PMID: 26362716]
[http://dx.doi.org/10.1177/1010428317698368] [PMID: 28459196]
![Wayfinder Image](/images/wayfinder.jpg)