![conference banner](/images/conference-banner.jpeg)
Abstract
Background: Melanoma causes more than 80% of deaths from all dermatologic cancers. Hence, screening and identifying effective compounds to inhibit the growth of melanoma have crucial importance in basic and clinical treatment.
Methods: High throughput screening was performed to screen and identify compounds that have anti-melanoma ability. Melanoma cell and mouse allograft models were used to examine the anti-tumor effects of Nuciferine (NCFR). Western blot, qPCR, and lentivirus overexpression were applied to detect the activation of the TLR4/NF-κB signaling pathway.
Results: NCFR administration significantly suppressed melanoma cell growth and tumor size by inhibiting the phosphorylation of p65. NCFR treatment also could suppress TNF-α-induced activation of NF-κB signaling. The anti-tumor effect of NCFR might be mediated by targeting Toll-like receptors 4.
Conclusion: NCFR inhibits melanoma cell growth and suppresses tumor size, which provides potential therapeutic strategies for melanoma treatment.
[http://dx.doi.org/10.1056/NEJMra052166] [PMID: 16822996]
[http://dx.doi.org/10.1016/S0140-6736(18)31559-9] [PMID: 30238891]
[http://dx.doi.org/10.1586/17469872.3.5.569] [PMID: 19649148]
[http://dx.doi.org/10.2165/11597560-000000000-00000] [PMID: 22668453]
[http://dx.doi.org/10.1038/ncomms11686] [PMID: 27221592]
[http://dx.doi.org/10.1073/pnas.1301218110] [PMID: 23898178]
[http://dx.doi.org/10.2147/OTT.S161109] [PMID: 29695914]
[http://dx.doi.org/10.1038/nri.2017.142] [PMID: 29379212]
[http://dx.doi.org/10.4049/jimmunol.165.7.3860] [PMID: 11034392]
[http://dx.doi.org/10.1038/sj.onc.1206520] [PMID: 12813457]
[http://dx.doi.org/10.1038/onc.2009.131] [PMID: 19483731]
[http://dx.doi.org/10.1128/MCB.19.8.5785] [PMID: 10409765]
[http://dx.doi.org/10.1128/MCB.19.9.5923] [PMID: 10454539]
[http://dx.doi.org/10.1074/jbc.274.43.30353] [PMID: 10521409]
[http://dx.doi.org/10.1006/smim.2000.0210] [PMID: 10723801]
[http://dx.doi.org/10.1152/ajprenal.00590.2010] [PMID: 21937604]
[http://dx.doi.org/10.1016/j.fct.2009.09.033] [PMID: 19799955]
[http://dx.doi.org/10.1371/journal.pone.0063770] [PMID: 23691094]
[http://dx.doi.org/10.1038/aps.2010.172] [PMID: 21127497]
[http://dx.doi.org/10.1016/j.jep.2012.05.024] [PMID: 22633982]
[http://dx.doi.org/10.1016/j.ejphar.2014.11.035] [PMID: 25499818]
[http://dx.doi.org/10.18632/oncotarget.5285] [PMID: 26485753]
[http://dx.doi.org/10.1038/s41419-019-1644-8] [PMID: 31138783]
[http://dx.doi.org/10.1016/S0002-9440(10)64328-3] [PMID: 12759251]
[http://dx.doi.org/10.1046/j.1523-1747.2002.01725.x] [PMID: 12060384]
[http://dx.doi.org/10.1111/j.1600-0749.2006.00304.x] [PMID: 16524427]
[http://dx.doi.org/10.1111/cas.13540] [PMID: 29453896]
[http://dx.doi.org/10.1089/10799900252952217] [PMID: 12034025]
[http://dx.doi.org/10.1021/acs.jafc.6b03031] [PMID: 27718563]
[http://dx.doi.org/10.3389/fphar.2015.00238] [PMID: 26539118]
![Wayfinder Image](/images/wayfinder.jpg)