Title:In Silico Evaluation of Various Signal Peptides to Improve Secretion of Humulin Protein in E. coli Host
Volume: 18
Issue: 3
Author(s): Soudabe Kavousipour, Mahadi Barazesh, Shiva Mohammadi*, Meghdad Abdollahpour- Alitappeh, Shirzad Fallahi, Amir Shakarami and Zeynab Hajmohammadi
Affiliation:
- Department of Medical Biotechnology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah,Iran
Keywords:
Extracellular secretion, E. coli, humulin, In silico, signal peptide, yebF precursor.
Abstract:
Background: Escherichia coli host has been the workhorse for the production of heterologous
proteins due to simplicity of use, low cost, availability of various expression vectors, and a
plethora of knowledge on its genetic characteristics, but without a suitable signal sequence, this
host cannot be used for the production of secretory proteins. Humulin is a form of insulin used to
treat hyperglycemia caused by types 1 and 2 diabetes. To improve expression and make a straightforward
production of Humulin protein, we chose a series of signal peptides.
Objective: The aim of this study was to predict the most excellent signal peptides to express secretory
Humulin in E. coli organism.
Methods: Therefore, to forecast the most excellent signal peptides for expression of Humulin in
Escherichia coli, 47 signal sequences from bacteria organisms were elected and the most imperative
elements of them were studied. Hence, signal peptide probability along with physicochemical
features was evaluated by signal 4.1, and Portparam, PROSO II servers respectively. Later, the in-
-silico cloning in a known pET28a plasmid system also estimated the possibility of best signal peptide+
Humulin expression in E. coli.
Results: The outcomes demonstrated that among 47 signal peptides only 2 signal peptides can be
considered as suitable signal peptides.
Conclusion: Ultimately protein yebF precursor (YEBF_ECOLI) and protein yebF precursor
(YEBF_YERP3) were suggested as the most excellent signal peptides to express Humulin (With D
scores 0.812 and 0.623, respectively). Although verification of these results warrants experimental
analysis.