Title:Indirect Determination of Amikacin by Gold Nanoparticles as Redox Probe
Volume: 18
Issue: 6
Author(s): Mansureh Alizadeh, Mandana Amiri*Abolfazl Bezaatpour
Affiliation:
- Department of Chemistry, University of Mohaghegh Ardabili, Ardabil,Iran
Keywords:
Amikacin, gold nanoparticles, electrochemical sensor, voltammetry, antibutics, human serum.
Abstract:
Background: Amikacin is an aminoglycoside antibiotic used for many gram-negative bacterial
infections like infections in the urinary tract, infections in brain, lungs and abdomen. Electrochemical
determination of amikacin is a challenge in electroanalysis because it shows no voltammetric peak
at the surface of bare electrodes.
Objective: In this approach, a very simple and easy method for indirect voltammetric determination of
amikacin presented in real samples. Gold nanoparticles were electrodeposited at the surface of glassy
carbon electrode in constant potential.
Methods: The effect of several parameters such as time and potential of deposition, pH and scan rates
on signal were studied. The cathodic peak current of Au3+ decreased with increasing amikacin concentration.
Quantitative analysis of amikacin was performed using differential pulse voltammetry by following
cathodic peak current of gold ions.
Results: Two dynamic linear ranges of 1.0 × 10−8–1.0 × 10-7 M and 5.0 × 10−7–1.0 × 10-3 M were obtained
and limit of detection was estimated 3.0× 10−9 M.
Conclusion: The method was successfully determined amikacin in pharmaceutical preparation and
human serum. The effect of several interference in determination of amikacin was also studied.