Title:Phytochemical Analysis and Antidiabetic Potential of Armoracia Rusticana: Pharmacological and Computational Approach
Volume: 24
Issue: 3
Author(s): Anam Javaid, Usman Ali Ashfaq*, Zeeshan Zafar, Arina Akmal, Saman Taj and Hina Khalid
Affiliation:
- Department of Bioinformatics and Biotechnology, Government College University, Faisalabad,Pakistan
Keywords:
Anti-diabetic, phytochemicals, docking, alpha-glucosidase, enzyme inhibition assay, Armoracia rusticana.
Abstract:
Aims & Objective: Armoracia rusticana has high medicinal values and is an excellent
source of phytochemicals. This study was aimed to evaluate the antidiabetic potential of bioactive
compounds from Armoracia rusticana.
Methods: The antidiabetic analysis revealed that Armoracia rusticana was highly active against α-
glucosidase with IC50 values of 5.6 μg/ml. Furthermore, molecular docking was used to identify
the active constituents against α-glucosidase, while using acarbose as a controlled drug.
Results: Upon phytochemical screening, it was found that six out of ten phytochemicals were
successfully docked in the respective binding sites. The lead phytochemical was Quercetin 3-Obeta-
D-xylopyranoside, which displayed a more binding score as compared to acarbose. They were
subjected to analyze for drug-like properties, which further strengthen its validation.
Conclusion: It was, therefore, concluded that Armoracia rusticana might potentially be used in the
amelioration of type 2 diabetes. Potential molecules identified from this study could be considered
as a lead drug to cure diabetes mellitus.