Title:MiR-493 Induces Cytotoxic Autophagy in Prostate Cancer Cells through Regulation on PHLPP2
Volume: 21
Issue: 14
Author(s): Jun Deng, Ming Ma , Wei Jiang, Liangliang Zheng and Suping Cui *
Affiliation:
- Department of Urology, the First Affiliated Hospital of Nanchang University, Nanchang 330006,China
Keywords:
MiR-493, Prostate cancer, PHLPP2, autophagy, qRT-PCR, PC cells.
Abstract:
Background: MiR-493 promotes the proliferation of prostate cancer (PC) cells by targeting
PHLPP2. We aimed to explore the relationship between miR-493 and autophagy in PC.
Methods: qRT-PCR and western blotting were used to determine the mRNA levels and protein expression
of miR-493, PHLPP2, autophagy gene BECN1 and ATG7 in PC cells. The autophagy gene expression
was determined after PC cells transfected with miR-493 precursor or PHLPP2 precursor. Corresponding
changes of autophagy phenotype and PC cell function were also studied.
Results: The mRNA levels and protein expression of miR-493, PHLPP2, BECN1 and ATG7 in PC
cells were significantly decreased in PC cells. Overexpression of miR-493 or PHLPP2 markedly upregulated
the expression levels of BECN1 and ATG7 in PC cells. Overexpression of miR-493 and
PHLPP2 markedly promoted autophagy, and inhibited the invasion and cloning formation of PC cells.
Conclusion: MiR-493 is a potent inducer of cytotoxic autophagy that leads to prostate cancer inhibition
by regulating on PHLPP2.