A Neuroinflammation Inhibitor, Hypoxylon xanthone A, from Soil Fungus Hypoxylon sp.

Title:A Neuroinflammation Inhibitor, Hypoxylon xanthone A, from Soil Fungus Hypoxylon sp.

Volume: 17 Issue: 2

Author(s): Ying Xiao, Bing Xu, You Kang, Yuedi Li, Yong Cui*, Wei Liu and Zheng Xiang*

Affiliation:

• School of Medical Devices, Shenyang Pharmaceutical University, Shenyang,China

• School of Pharmaceutical Science, Liaoning University, Shenyang,China

Keywords: Soil fungus Hypoxylon sp., metabolite, NMR spectrum, ECD, chemical constituent, anti-neuroinflammatory.

Abstract: Hypoxylon sp. was used to ferment at 25°C for 45 days. The solid culture of Hypoxylon sp. was extracted with 75% EtOH under ultrasonic for twice. And the dried combined extracts were then suspended in H2O and partitioned with ethyl acetate. EtOAc extracts were subjected to a silica gel column and eluted with petroleum ether - acetone to afford seven fractions. Sephadex LH-20 and RPHPLC were used subsequently to yield a novel xanthone metabolite (Hypoxylon xanthone A). Its structure was elucidated based on HR-ESI-MS, 1D-, 2D-NMR spectra, and the comparison of the experimental and calculated ECD spectra. The anti-neuroinflammatory assay of Hypoxylon xanthone A, as manifested by the inhibitory effect on LPS-induced NO production in BV-2 microglial cells, indicated almost the same inhibitory effect as minocycline in a dose-dependent manner within the concentration of 1-50 μM, suggesting that Hypoxylon xanthone A could be a new potential neuroinflammation inhibitor.

Cite this article as:

Xiao Ying, Xu Bing , Kang You , Li Yuedi , Cui Yong *, Liu Wei and Xiang Zheng *, A Neuroinflammation Inhibitor, Hypoxylon xanthone A, from Soil Fungus Hypoxylon sp., Letters in Organic Chemistry 2020; 17 (2) . https://dx.doi.org/10.2174/1570178616666191023100252