Abstract
Cefotaxime sodium has an antibacterial effect and it is classified as a third-generation broad spectrum cephalosporin antibiotic. It is irreversible, oxidized at the glassy carbon electrode in various buffer systems and at different pH values. The detailed mechanism of the oxidation process is described in this manuscript. Differential pulse and square wave voltammetric methods were developed for its determination in pharmaceutical dosage forms and spiked human serum samples according to the linear relation between the peak current and cefotaxime sodium concentration. For analytical purposes, a very well resolved diffusion controlled voltammetric peak was obtained in Britton-Robinson buffer at pH 2.0 at 0.87 and 0.89V for differential pulse and square wave voltammetric techniques, respectively. The linear response was obtained within the range of 1x10 – 6 – 6x10 – 5 M with a detection limit of 2.83x10 – 7 M for differential pulse and 2x10 – 6 – 6x10 – 5 M with a detection limit of 3.61x10 – 7 M for square wave voltammetric techniques. The repeatability and reproducibility of the methods for both media (supporting electrolyte and serum sample) were determined. Precision and accuracy of the developed method were used for the recovery studies. The standard addition method was used for the recovery studies. No electroactive interferences were found in biological fluids from the endogenous substances and additives present in pharmaceutical dosage form.
Keywords: Cefotaxime, Pharmaceuticals, Voltammetry, Biological samples, Oxidation, Determination
Current Pharmaceutical Analysis
Title: Anodic Oxidation of Antibacterial Drug Cefotaxime Sodium and its Square Wave and Differential Pulse Voltammetric Determination in Pharmaceuticals and Human Serum
Volume: 5 Issue: 2
Author(s): Burcu Dogan, Aysegul Golcu, Mustafa Dolaz and Sibel A. Ozkan
Affiliation:
Keywords: Cefotaxime, Pharmaceuticals, Voltammetry, Biological samples, Oxidation, Determination
Abstract: Cefotaxime sodium has an antibacterial effect and it is classified as a third-generation broad spectrum cephalosporin antibiotic. It is irreversible, oxidized at the glassy carbon electrode in various buffer systems and at different pH values. The detailed mechanism of the oxidation process is described in this manuscript. Differential pulse and square wave voltammetric methods were developed for its determination in pharmaceutical dosage forms and spiked human serum samples according to the linear relation between the peak current and cefotaxime sodium concentration. For analytical purposes, a very well resolved diffusion controlled voltammetric peak was obtained in Britton-Robinson buffer at pH 2.0 at 0.87 and 0.89V for differential pulse and square wave voltammetric techniques, respectively. The linear response was obtained within the range of 1x10 – 6 – 6x10 – 5 M with a detection limit of 2.83x10 – 7 M for differential pulse and 2x10 – 6 – 6x10 – 5 M with a detection limit of 3.61x10 – 7 M for square wave voltammetric techniques. The repeatability and reproducibility of the methods for both media (supporting electrolyte and serum sample) were determined. Precision and accuracy of the developed method were used for the recovery studies. The standard addition method was used for the recovery studies. No electroactive interferences were found in biological fluids from the endogenous substances and additives present in pharmaceutical dosage form.
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Cite this article as:
Dogan Burcu, Golcu Aysegul, Dolaz Mustafa and Ozkan A. Sibel, Anodic Oxidation of Antibacterial Drug Cefotaxime Sodium and its Square Wave and Differential Pulse Voltammetric Determination in Pharmaceuticals and Human Serum, Current Pharmaceutical Analysis 2009; 5 (2) . https://dx.doi.org/10.2174/157341209788172861
DOI https://dx.doi.org/10.2174/157341209788172861 |
Print ISSN 1573-4129 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-676X |
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