Title:Bioorthogonal Photoclick 1,3-Dipolar Cycloaddition Reaction on Protein – A Review
Study
Volume: 27
Issue: 4
Author(s): Pritha Mandal*
Affiliation:
- Department of Chemistry, Krishnagar Government College, Krishnagar, West Bengal, 741101, India
Keywords:
Photoclick reaction, bioorthogonal reaction, protein labeling, dipolar cycloaddition, tetrazole, SPAAC.
Abstract: Click reactions have gained enormous popularity among chemists for their ambient
reaction parameters and wide application in various frontier research fields. Photo-initiated
click reactions add another dimension of spatiotemporal control which ensures fruitful
bioorthogonal reaction. Several cycloaddition reactions, enlisted in the category of ‘photo
click’ reactions due to the fast kinetics and ambient reaction conditions, are widely utilized by
scientists for bioorthogonal conjugation. In this review, various types of 1,3-dipolar cycloaddition
reactions and their applications in the field of protein bioconjugation are discussed.