Next Generation Sequencing (NGS) provides researchers with an
unprecedented opportunity to produce a large volume of DNA sequences quickly, and
is one of the fundamental methods for high-throughput genomic studies. Currently, the
most widely-used NGS platforms are Illumina, Roche 454 and SB SOLiD. These
platforms differ in the chemistry used in the sequencing process and the length of
sequencing read generated. Each platform has its own strengths and weaknesses. In
particular, the required length of the sequence read to be generated plays an important
role when designing an experiment. For example, a longer read length would be needed
in the assembly of a novel genome, while throughput-maximizing PED-based
techniques would be better-suited when shorter reads will suffice.
Keywords: ChIP-Seq, Galaxy, RNA-Seq.
