Transcriptome analysis, or transcriptome sequencing, concerns the
transcript sequences transcribed from the genome of a specific cell type at specific time
and growth conditions. Previous studies have clearly demonstrated that, besides
messenger RNA (mRNA), the transcribed RNA sequences also contain large amounts
of ribosomal RNA (rRNA), transfer RNA (tRNA) and small-sized non-coding RNA
(ncRNA). Transcriptome analysis focuses mainly on mRNA, and sometimes, certain
types of ncRNA species which may be co-isolated with mRNA when gene expression
and regulation are of the major concern. From transcriptome sequencing, a number of
biological information can be retrieved. These include gene expression level,
transcriptome landscape across the entire genome, Gene Ontology, pathway, etc.
Notice that transcriptome analysis normally refers to the whole transcriiptome analysis
of a cell population. The result is in fact a combination of millions of potentially
diversified single-cell transcriptomes.
Keywords: Gene Ontology, GO, KEGG, Pathway, RPKM, Transcriptome.
