Cell and enzyme immobilization is a widely employed technique in many industrial
applications. Over the recent years, researchers have been also focusing on benefiting from the merits of
immobilization techniques in biohydrogen studies. In this chapter, various immobilization techniques
and their application results in terms of enhancing biohydrogen production via different processes,
namely anaerobic dark and light fermentation, are presented. For this purpose, firstly, principles of
various immobilization techniques and protocols are discussed and secondly the literature reports on the
results of use of immobilization techniques are reviewed in regards to their biohydrogen productions.
Keywords: Immobilization, bioreactor, biohydrogen, biofilm systems, immobilization materials, carrier
material, fixed bed, fluidized bed, expended bed, UASB.
