Immunoaffinity Chromatography (IAC) is a type of liquid chromatography that uses the
highly specific binding of antibodies for the retention of a target. This approach can be used in a myriad
of techniques for the selective purification, concentration and/or analysis of target compounds. This
chapter discusses the basic components of an IAC method, including the support, stationary phase,
immobilization technique, and proper sample application and elution conditions. Several applications
of IAC are also considered. These applications include immunoextraction, immunodepletion,
chromatographic immunoassays, and post-column immunodetection. A wide range of targets that have
been isolated or measured by these techniques are listed. Related methods based on antibody mimics
are also discussed.
Keywords: Antibodies, Immunoaffinity Chromatography
