2-Deoxy-D-glucose is an important pharmaceutical intermediate, and its
analytical characterization is critical for establishing its purity and quality. This chapter
summarizes spectroscopic techniques, including UV, IR, NMR, and mass
spectrometry, along with HPLC and GC studies used for the complete structural
elucidation and purity analysis of 2-Deoxy-D-glucose. The UV spectrum of 2-DeoxyD-Glucose showed no distinct peaks. The IR spectrum displayed characteristic bands
for the O-H and C-H functional groups. 1H, 13C, APT, DEPT NMR, HSQC, and HMBC
experiments confirmed the nominally proposed structure. ESI-MS revealed an [M+Na]+
ion at m/z 187. Specific optical rotation was measured in water. HPLC studies
estimated the related substances and assay to be 1.2% and 99.8%, on an anhydrous
basis, respectively. The residual solvents, such as methanol, isopropyl alcohol, ethyl
acetate, and toluene, were determined by GC headspace and found to be within the
limits. The collective analytical evidence confirmed that the test sample met the quality
specifications for 2-Deoxy-D-glucose.
Keywords: Assay, Chromatography, Characterization, Karl fisher, Related substances, Spectroscopy, Specific optical rotation, 2-deoxy-D-glucose.
