Besides qualitative and quantification of nucleic acids, purification is an
integral part of any genetic engineering experiments. In genetic engineering
experiments, the purity of nucleic acid matters the most. Nucleic acid purification can
be achieved by the phenol-chloroform method, alcohol-based purification, agarose gel
electrophoresis, and chromatographic purification. These purifications are based on the
interactions between bases of nucleic acids and chemical reagents. In this chapter,
nucleic acid purification is explained in detail.
Keywords: Alcohol, Agarose gel electrophoresis, Chromatography, Genetic engineering, Nucleic acid, Phenol-chloroform.
