Bacopa monnieri (L.) Wettstein is a medicinal herb from the family
Plantaginaceae widely known as ‘water hyssop’ or ‘brahmi’. The therapeutic potential
of plants is due to the presence of many bioactive secondary metabolites, majorly
brahmine, herpestine, alkaloids, and saponins (bacosides), which are responsible for
pharmacological effects including neuroprotective, hepatoprotective, gastroprotective,
antioxidant, anti-inflammatory, and antimicrobial properties. Vegetative cultivation of
Bacopa on a large scale has its limitations due to the lack of viability of seeds during
propagation and the unpredictable nature of the production of phytochemicals for
commercial purposes, which can be overcome by tissue culture mechanism. Over the
past few decades, many studies on the tissue culture of Bacopa in establishing a
standardized protocol were reported. This chapter deals with de novo organogenesis of
the root and shoot along with the callus induction and somatic embryogenesis from
different explants of B. monnieri on MS basal nutrient medium supplemented with
Plant Growth Regulators.
Keywords: Bacopa monnieri, Micropropagation, Plant growth regulator.
