Aloe vera is a medicinal plant with several properties and is used in
pharmaceutical, medicinal, biomaterials, food, and cosmetic industries. This plant is
associated with hot climates and dry habitats, with plantlet production based on lateral
shoot propagation, an expansive and slow method, insufficient to meet the increasing
demand of the industry. Therefore, the development of a suitable Aloe vera
micropropagation protocol is crucial, keeping the genetic integrity and providing largescale plantlets production. Nevertheless, parameters like source of plant tissue, surfacesterilization process, culture medium conditions and plant growth regulator
concentration can affect the morphogenic response process. Since all parameters are
defined to obtain the best performance, the micropropagation protocol is suitable to be
used commercially, providing mass production of Aloe vera plantlets with high quality.
Keywords: Aloe vera, micropropagation, medicinal plant.