In Vitro Applications of Drug-carrying Nanoparticle Systems in Cell Culture Studies

Pp: 376-403 (28)

* (Excluding Mailing and Handling)

Abstract

The safety and efficacy of each drug candidate, including nanomedicine considered for pharmaceutical use, primarily must be determined in vitro. In this context, the most widely used method is cytotoxicity tests, which include cell culture studies. It examines the parameters of membrane integrity, metabolite incorporation, structural alteration, survival and growth in tissue culture, enzyme assays, and the capacity for transplantation within the scope of viability tests. Within the scope of cell culture studies, tests related to apoptosis, which are effective in proper cell cycle, immune system and embryonic development, are also included. Another way to detect cell viability is to detect the biomolecules it expresses. Determination of protein expression is one of the preferred methods in this sense. Within the scope of this chapter, there is information about cell culture-based methods under these main subjects, which are applied to nanomedicines.

Keywords: Annexin V, Apoptosis assay, Capture antibody, Cell culture, Colorimetric assay, Comet assay, Cytotoxicity, DNA Fragmentation, Enzyme-linked Immunosorbent Assay, Flow cytometry, Fluorescence-activated cell sorting, Fluorometric assays, Luminometric assay, Microtiter plate, Multiplex ELISA Polyvinylidene difluoride, Propidium iodide, Real-time viability assay, SDS-PAGE Electrophoresis, Western Blot.