The safety and efficacy of each drug candidate, including nanomedicine
considered for pharmaceutical use, primarily must be determined in vitro. In this
context, the most widely used method is cytotoxicity tests, which include cell culture
studies. It examines the parameters of membrane integrity, metabolite incorporation,
structural alteration, survival and growth in tissue culture, enzyme assays, and the
capacity for transplantation within the scope of viability tests. Within the scope of cell
culture studies, tests related to apoptosis, which are effective in proper cell cycle,
immune system and embryonic development, are also included. Another way to detect
cell viability is to detect the biomolecules it expresses. Determination of protein
expression is one of the preferred methods in this sense. Within the scope of this
chapter, there is information about cell culture-based methods under these main
subjects, which are applied to nanomedicines.
Keywords: Annexin V, Apoptosis assay, Capture antibody, Cell culture, Colorimetric assay, Comet assay, Cytotoxicity, DNA Fragmentation, Enzyme-linked Immunosorbent Assay, Flow cytometry, Fluorescence-activated cell sorting, Fluorometric assays, Luminometric assay, Microtiter plate, Multiplex ELISA Polyvinylidene difluoride, Propidium iodide, Real-time viability assay, SDS-PAGE Electrophoresis, Western Blot.
