The embryonic culture system is the main part of in-vitro embryonic
development. The culture system consists of chemical factors such as the chemical
composition of the culture media and physical factors such as temperature, pH, oil
overlay and, incubation systems. The optimal culture conditions are needed to support
embryonic development in-vitro, increase pregnancy, implantation rates, and decrease
the level of pregnancy loss. Any suboptimality in culture conditions may impair
embryonic development and affect subsequent viability. In this chapter, we will focus
on the current culture system in the field of assisted reproductive technology (ART),
passing by current culture media formulations, deficiencies, culture devices used, and
the areas that need further investigations.
Keywords: Culture dishes, Culture media, Culture system, Embryo culture, Oil overlay, pH.
